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ProteomicsWestern blot validation - The goal is to define guidelines for Ab western blot validation
The goal is to define guidelines for Ab western blot validation
ORGANISM(S): Homo sapiens (Human) 
2018-08-21 | PXD005914 | Pride
Wester blot Tmt Prm Transcriptomics 
TranscriptomicsExpression data from Control diet, Western diet and Western diet + DHA fed rats.
LV hypertrophy is associated with Western diet consumption, while intake of n-3 polyunsaturated fatty acids is associated with anti-hypertrophic effects. We treated rats for 12 weeks with either a Control diet, a Western diet or a Western + DHA diet. For each of the 3 dietary treatments there were...
ORGANISM(S): Rattus norvegicus 
2013-11-22 | E-GEOD-52603 | biostudies-arrayexpress
ProteomicsOvercoming off-targets: Assessing western blot signals for Bcnt/Cfdp1, a tentative component of the chromatin remodeling complex
The mammalian Bucentaur/Craniofacial developmental protein 1 (Bcnt/Cfdp1) is a tentative component of the Srcap (SNF2-Related CBP Activator Protein) chromatin remodeling complex, but little is known about its properties, partly because few antibodies are available to detect the endogenous protein.　W...
ORGANISM(S): Mus musculus (Mouse) 
2020-08-03 | PXD019016 | Pride
Bcnt 
UnknownAntigen retrieval by citrate solution improves western blot signal.
Not available
S-EPMC6423990 | biostudies-literature
TranscriptomicsA Multi-loop, Double-cube Microarray Design Applied to Prostate Cancer Cell Lines with Variable Sensitivity to Histone Deacetylase Inhibitors
Implementation of a complex design for a microarray experiment on resistance mechanisms of histone deacetylase inhibitors (HDACI). To improve our understanding of the underlying mechanism of HDACI resistance in prostate cancer cells, we designed a novel ‘‘multiple-loop, double-cube’’ cDNA microarray...
ORGANISM(S): Homo sapiens 
2011-12-17 | E-GEOD-34452 | biostudies-arrayexpress
UnknownImproving rigor and reproducibility in western blot experiments with the blotRig analysis.
Not available
S-EPMC11405887 | biostudies-literature
MetabolomicsBerberine improves intestinal barrier and cognitive impairment by remodeling intestinal microbiota in C57BL/6 septic mice induced by LPS
After feeding LPS induced C57BL/6 septic mice with berberine, we performed behavioral, HE staining, ELISA, western blot, metagenomic sequencing of gut microbiota and untargeted metabolomics analysis to evaluate the effects of berberine on septic mice.
2026-06-17 | MTBLS14775 | MetaboLights
UnknownBlind spots on western blots: Assessment of common problems in western blot figures and methods reporting with recommendations to improve them.
Not available
S-EPMC9518894 | biostudies-literature
UnknownMisleading Westerns: Common Quantification Mistakes in Western Blot Densitometry and Proposed Corrective Measures.
Not available
S-EPMC6360618 | biostudies-literature
UnknownIn Situ Single-Cell Western Blot on Adherent Cell Culture.
Not available
S-EPMC6759404 | biostudies-literature
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