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ProteomicsCombined 15N-Labelling and TandemMOAC Quantifies Phosphorylation of MAP Kinase Substrates Downstream of MKK7 in Arabidopsis
Reversible protein phosphorylation is a widespread posttranslational modification that plays a key role in eukaryotic signal transduction. Due to the large dynamic range of protein abundance, low stoichiometry and transient nature of protein phosphorylation, the detection and accurate quantification...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2018-01-02 | PXD008200 | Pride
Phosphoproteomics Tandem moac 15n labeling Mapk Mpk substrate 
ProteomicsArabidopsis, protein turnover, plastid, leaf growth and development-15N progressive labelling
In order to characterize plant protein degradation rates and understand their determinants as they relate to plant growth rate, we have applied 15N labelling approaches in leaves of the Arabidopsis rosette. We found a series of leaves in Arabidopsis plants for which the proteome was stable over time...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2017-02-01 | PXD004550 | Pride
Arabidopsis Development Plastid Protein turnover Leaf growth 
ProteomicsTo invesigate the role of autophagy in Arabidopsis root cellular protein turnover and proteostasis (15N Spike-in root)
Protein turnover analysis using partial 15N labelling and protein abundance analysis using 15N spike-in were coupled to investiage the role of autophagy in protein degradation and proteostasis. Our findings show that dysfuncional autophagy due to loss-of-function autophagy components ATG5 and 11 lea...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-07-12 | PXD010992 | Pride
Arabidopsis 15n labelling Protein turnover Autophagy 
ProteomicsArabidopsis, protein turnover, plastid, leaf growth and development-15N fully labelling as reference
In order to characterize plant protein degradation rates and understand their determinants as they relate to plant growth rate, we have applied 15N labelling approaches in leaves of the Arabidopsis rosette. We found a series of leaves in Arabidopsis plants for which the proteome was stable over time...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2017-02-01 | PXD004549 | Pride
Arabidopsis Development Plastid Protein turnover Leaf growth 
ProteomicsTo invesigate the role of autophagy in Arabidopsis shoot cellular protein turnover and proteomstasis (15N Spike-in shoot)
Protein turnover analysis using partial 15N labelling and protein abundance analysis using 15N spike-in were coupled to investiage the role of autophagy in protein degradation and proteostasis. Our findings show that dysfuncional autophagy due to loss-of-function autophagy components ATG5 and 11 lea...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-10-13 | PXD010948 | Pride
Arabidopsis Protein turnover 15n spike-in Autophagy 
ProteomicsTo invesigate the role of autophagy in Arabidopsis shoot cellular protein turnover and proteostasis, part 2
Protein turnover analysis using partial 15N labelling and protein abundance analysis using 15N spike-in were coupled to investiage the role of autophagy in protein degradation and proteostasis. Our findings show that dysfuncional autophagy due to loss-of-function autophagy components ATG5 and 11 lea...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-07-12 | PXD010932 | Pride
Arabidopsis 15n labelling Protein turnover Autophagy 
ProteomicsTo invesigate the role of autophagy in Arabidopsis root cellular protein turnover and proteostasis
Protein turnover analysis using partial 15N labelling and protein abundance analysis using 15N spike-in were coupled to investiage the role of autophagy in protein degradation and proteostasis. Our findings show that dysfuncional autophagy due to loss-of-function autophagy components ATG5 and 11 lea...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-07-12 | PXD010900 | Pride
Arabidopsis 15n labelling Protein turnover Autophagy 
UnknownN uptake, assimilation and isotopic fractioning control δ 15N dynamics in plant DNA: A heavy labelling experiment on Brassica napus L.
Not available
S-EPMC7951814 | biostudies-literature
MetabolomicsPantothenate biosynthesis is critical for chronic infection by the neurotropic parasite Toxoplasma gondii
Coenzyme A (CoA) is an essential molecule acting in metabolism, post-translational modification, and regulation of gene expression. While all organisms synthesize CoA, many, including humans, are unable to produce its precursor, pantothenate. Intriguingly, like most plants, fungi and bacteria, paras...
2022-03-21 | MTBLS2501 | MetaboLights
ProteomicsThylakoid membranes from Arabidopsis LC-MSMS
The small heat shock protein (sHsp) chaperones are crucial for cell survival and can prevent aggregation of client proteins that partially unfold under destabilizing conditions. Most investigations on the chaperone activity sHsps are based on a limited set of thermosensitive model substrate client ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2017-06-21 | PXD006734 | Pride
Hsp21 Arabidopsis Thermomemory Photosynthesis Thylakoid Stable isotope labelling Stress response Quantitative mass spectrometry Chaperone Heat shock protein Membranes 
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