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ProteomicsDifferential Enzymatic 16O/18O Labelling for the Detection of Cross-Linked Nucleic Acid-Protein Heteroconjugates
Enzymatic differential 16O/18O labelling of nucelic acid-protein heteroconjugates. Heteroconjugates are identified against a complex background using an automated analysis workflow.
ORGANISM(S): Bos taurus (Bovine) 
2017-09-11 | PXD003673 | Pride
Lc-ms Cross-linking Nucelic acid-protein heteroconjugates 
GenomicsDeep sea red clay 18O-SIP experiment
Deep sea red clay 18O-SIP experiment Raw sequence reads
PRJNA473406 | ENA
Unknown18O kinetic isotope effects in non-heme iron enzymes: probing the nature of Fe/O2 intermediates.
Not available
S-EPMC2788235 | biostudies-literature
ProteomicsSCX, 18O-labeling and charge reversal for C-terminomics
The proteome wide, mass spectrometry based identification of protein C-termini is hampered by factors such as poor ionization efficiencies, low yields in labeling strategies or the need for enrichment procedures. We present a bottom-up proteomics workflow to identify protein C-termini utilizing a co...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2019-06-20 | PXD013486 | Pride
Lc-ms Charge-reversal derivatization C-terminomics 
ProteomicsValidation of a novel shotgun proteomic workflow for the discovery of protein-protein interactions: focus on ZNF521
The study of protein-protein interactions is increasingly relying on mass spectrometry (MS). The classical approach of separating immunoprecipitated proteins by SDS-PAGE followed by in-gel digestion is long and labour-intensive. Besides, it is difficult to integrate it with most quantitative MS-base...
ORGANISM(S): Homo sapiens (Human) 
2015-04-07 | PXD001609 | Pride
Ap-ms  interactomics  18o labelling  znf521  znf423  spt16  spt5  nurd core complex  proteomics  mass spectrometry  shotgun proteomics 
TranscriptomicsIdentification of PM-localized proteins
In order to assess the quality of alleged PM identifications from Arabidopsis, PM-enriched fractions were compared to PM-depleted fractions using 18O isotopic labeling and mass spectrometry. The two samples submitted are biological replicates. Keywords: Protein Localization via MS Two biological rep...
ORGANISM(S): Arabidopsis thaliana 
2005-06-21 | E-GEOD-2830 | biostudies-arrayexpress
ProteomicsProbing endogenous peptide acceptor substrate specificities of TbSTT3A and TbSTT3B
We performed glycoproteomics on endogenous parasite glycoproteins using sequential endoglycosidase-H and peptide-N-glycosidase-F digestions, the latter in the presence of H2[18O]. This allowed us to assess the relative occupancies of native N-glycosylation sites by endoglycosidase-H resistant N-glyc...
ORGANISM(S): Trypanosoma brucei 
2017-09-20 | PXD007267 | Pride
Trypanosoma Glycoproteomics Oligosaccharyltransferase N-linked glycosylation Stt3 Glycoprotein biosynthesis Parasitology 
UnknownMethodology and validation of a new tandem mass spectrometer method for the quantification of inorganic and organic 18O-phosphate species.
Not available
S-EPMC7039501 | biostudies-literature
Unknown18O-Tracer Metabolomics Reveals Protein Turnover and CDP-Choline Cycle Activity in Differentiating 3T3-L1 Pre-Adipocytes.
Not available
S-EPMC4898700 | biostudies-literature
ProteomicsQuantitative analysis of in vivo methionine oxidation of the human proteome
A novel stable isotope labelling strategy was developed to quantify methionine oxidation in an unstressed human proteome. Cell extracts were oxidized with 18O labelled hydrogen peroxide following cell lysis in order to convert all unoxidized methionine residues to an oxidized version with a heavy l...
ORGANISM(S): Homo sapiens (Human) 
2019-12-09 | PXD014629 | Pride
Methionine oxidation Unstressed proteome Human cell line 
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