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ProteomicsAnimal product-free culture conditions for 3D primary human hepatocyte spheroids improve long-term viability and drug metabolism
3D cultures of primary human hepatocytes (3D PHH) are successfully used to reduce and replace the use of animal experiments in biomedical research. Yet, the initial formation of 3D PHH is highly dependent on the supplementation with fetal bovine serum (FBS). However, the molecular composition of FBS...
ORGANISM(S): Homo sapiens (Human) 
2026-01-23 | PXD056428 | Pride
Human Setum-free Fbs Liver 3d phh 
ProteomicsOCT1 (SLC22A1) transporter kinetics and regulation in primary human hepatocyte 3D spheroids
3D spheroids of primary human hepatocytes (3D PHH) keep a differentiated phenotype with retained metabolic function and proteome fingerprint for weeks in culture. As a result, 3D PHH are gaining ground as a model for mechanistic studies on liver homeostasis and in vitro to in vivo (IVIV)predictions...
ORGANISM(S): Homo sapiens (Human) 
2024-08-10 | PXD045733 | Pride
Human Hepatocytes Adme Liver 
TranscriptomicsTargeted RNA-seq (TempO-Seq) of temporal concentration responses to cosmetic ingredients exposures in PHH and HepaRG cells
For the purpose of mechanism-based risk assessment, we investigated temporal concentration-dependent responses in cultures of PHH and HepaRG cells exposed to three cosmetics ingredients, 2,7-naphthalenediol (NPT), triclosan (TCS) and butylated hydroxytoluene (BHT), that are suspected to have liver i...
ORGANISM(S): synthetic construct 
2024-03-01 | E-MTAB-12677 | biostudies-arrayexpress
TranscriptomicsTargeted RNA-seq (TempO-Seq) of temporal concentration responses to drug exposures in PHH and HepaRG cells
For the purpose of mechanism-based risk assessment, we investigated temporal concentration-dependent responses in cultures of PHH and HepaRG cells exposed to three drugs, acetaminophen (APAP), cyclosporine A (CSA) and valproic acid (VPA), that have a high liability for drug-induced liver injury. To ...
ORGANISM(S): synthetic construct 
2024-03-01 | E-MTAB-12668 | biostudies-arrayexpress
ProteomicsConditions for maintenance of hepatocyte differentiation and function in 3D cultures
3D spheroid cultures of primary human hepatocytes (PHH) are used in studies of hepatic drug metabolism and toxicity. However, the 3D spheroids are maintained under different conditions, with possible cofounding results. Here we performed an in-depth analysis of how various culture conditions influen...
ORGANISM(S): Homo sapiens (Human) 
2022-02-17 | PXD024632 | Pride
Primary human hepatocyte culture Lc-ms/ms 
UnknownOCT1 (SLC22A1) transporter kinetics and regulation in primary human hepatocyte 3D spheroids.
Not available
S-EPMC11283471 | biostudies-literature
UnknownComparison of Hepatic 2D Sandwich Cultures and 3D Spheroids for Long-term Toxicity Applications: A Multicenter Study.
Not available
S-EPMC5888952 | biostudies-literature
UnknownLong-term 3D cell culture models for hepatitis B virus studies.
Not available
S-EPMC12017837 | biostudies-literature
GenomicsInvestigating the effect of inflammatory conditions on acetaminophen-induced liver injury using 3D human multicellular microtissues
Investigating the effect of inflammatory conditions on acetaminophen-induced liver injury using 3D human multicellular microtissues
PRJNA413194 | ENA
Unknown3D microfluidic liver cultures as a physiological preclinical tool for hepatitis B virus infection.
Not available
S-EPMC5813240 | biostudies-literature
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