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Profiling 4-thiouridine modification present at position 8 of E. coli transfer RNAs
ORGANISM(S): Escherichia coli 
2022-01-30 | PXD028405 | Pride
We applied 4-thiouridine to cultured cells expressing the FLAG/HA-tagged RNA-binding proteins (RBPs) followed by UV 365 nm irradiation. The crosslinked RNA-protein complexes were isolated by immunoprecipitation, and the covalently bound RNA was partially digested with RNase T1 and radiolabeled. The ...
ORGANISM(S): Homo sapiens 
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