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Effect of 4sU labeling durations on quantification bias in nucleotide conversion RNA-seq data
Effect of 4sU labeling concentrations on quantification bias in nucleotide conversion RNA-seq data
To investigate the effect of increasing 4sU labeling concentrations on quantification bias in nucleotide conversion RNA-seq, we labeled 3 different cell lines with increasing concentrations of 4sU. We then measured 4sU dropout in 4sU samples compared to 4sU naive samples.
ORGANISM(S): Homo sapiens 
2024-02-06 | GSE229504 | GEO
To investigate the effect of labeling durations on quantification bias in nucleotide conversion RNA-seq, we labeled NIH cells with 4sU. We then measured 4sU dropout in 4sU samples compared to 4sU naive samples.
ORGANISM(S): Mus musculus 
2024-02-06 | GSE229506 | GEO
Mechanisms used by positive-stranded RNA viruses to control the translation-replication switch remain largely unexplored. Using metabolic labeling coupled with quantitative proteomics analyses, we unraveled the protein composition of temporal ribonucleoprotein complexes (RNPs) isolated during early ...
ORGANISM(S): Homo sapiens (Human) Enterovirus A71 
2025-06-04 | PXD036890 | Pride
Noncoding variants play a central role in the genetics of complex traits, but we still lack a full description of the main molecular pathways through which they act. Here we used molecular data to quantify the contribution of cis-acting genetic effects at each major stage of gene regulation from chr...
ORGANISM(S): Homo sapiens 
Genomics
Pulse chase 4SU labeling - DCP1a and DCP1b KO cells
We evaluated changes in mRNA stability and transcription using 4sU metabolic pulse labeling across a four hour time course following activation of Jurkat T cells with PMA and PHA Measurement of total mRNA (T) and 4sU labeled mRNA (IP) in three biological replicates at five time points: prior to acti...
ORGANISM(S): Homo sapiens 
Effect of 4sU labeling on quantification bias in nucleotide conversion RNA-seq data
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