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ProteomicsIntegrative analysis of genomics and proteomics data on clinical breast cancer tissue specimens extracted with acid guanidinium thiocyanate-phenol-chloroform
Acid guanidinium thiocyanate, phenol and chloroform extraction (AGPC) is a commonly used procedure to extract RNA from fresh frozen tissues and cell lines. In addition, DNA and proteins can be recovered, which makes AGPC an attractive source for integrative analysis on tissues of which little materi...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-29 | MSV000080789 | MassIVE
ProteomicsIntegrative analysis of genomics and proteomics data on clinical breast cancer tissue specimens extracted with acid guanidinium thiocyanate-phenol-chloroform
Acid guanidinium thiocyanate, phenol and chloroform extraction (AGPC) is a commonly used procedure to extract RNA from fresh frozen tissues and cell lines. In addition, DNA and proteins can be recovered, which makes AGPC an attractive source for integrative analysis on tissues of which little materi...
ORGANISM(S): Homo sapiens (Human) 
2016-06-24 | PXD001533 | Pride
Proteogenomics Acid guanidinium phenol-chloroform Shotgun proteomics Rna bee Breast cancer Sample preparation 
UnknownApplicability of polyethylene glycol precipitation followed by acid guanidinium thiocyanate-phenol-chloroform extraction for the detection of SARS-CoV-2 RNA from municipal wastewater.
Not available
S-EPMC7568484 | biostudies-literature
TranscriptomicsTranswell-cultured and miRNAs-transfected T84 cells
MicroRNAs are small non-coding RNA species, some of which are playing important roles in cell differentiation. However, the level of participations of microRNAs in epithelial cell differentiation is largely unknown. Here, we found that expression levels of four microRNAs (miR-210, miR-338-3p, miR-33...
ORGANISM(S): Homo sapiens 
2009-07-14 | E-GEOD-15385 | biostudies-arrayexpress
TranscriptomicsKYSE170 transfected with ncRNA or miR-210
MicroRNAs are small non-coding RNA species, some of which are playing important roles in malignancies. However, roles of miR-210 in cancer have yet to be unknown and controversial. Here, we found the expression level of miR-210 is downregulated in human esophageal squamous cell carcinoma and its cel...
ORGANISM(S): Homo sapiens 
2011-01-05 | E-GEOD-20637 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of rat duodenum during immobilization
In the present study, we examined duodenal gene expression in rats subjected to immobilization, to elucidate the mechanism of the stress response in the duodenum. Experiment Overall Design: Total RNA was extracted from the duodenal mucosa with an acid guanidinium thiocyanate-phenol-chloroform mixtur...
ORGANISM(S): Rattus norvegicus 
2008-06-13 | E-GEOD-4426 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of mouse rhythmic mRNA expression in the distal colon
To identify a cohort of rhythmically expressed genes in the murine Distal Colon,microarrays were used to measure gene expression over a 24-hour light/dark cycle.The rhythmic transcripts were classified according to expression patterns, functions and association with physiological and pathophysiologi...
ORGANISM(S): Mus musculus 
2008-10-25 | E-GEOD-10644 | biostudies-arrayexpress
TranscriptomicsRNA-seq transcriptome profiling of the Southern Ocean diatom Fragilariopsis cylindrus under different experimental conditions, silica limitation and two different light spectra
The Southern Ocean diatom Fragilariopsis cylindrus was grown under half-saturating concentrations of silicate (0.3 uM) and in the blue (480 - 540 nm) and red (550 - 700 nm) light spectrum to investigate gene expression responses using RNA-seq relative to optimal growth conditions (deposited under ac...
ORGANISM(S): Fragilariopsis cylindrus 
2020-12-31 | E-MTAB-5429 | biostudies-arrayexpress
UnknownFeasibility of Phosphoproteomics on Leftover Samples After RNA Extraction With Guanidinium Thiocyanate.
Not available
S-EPMC8111777 | biostudies-literature
UnknownHigh Efficiency RNA Extraction From Sperm Cells Using Guanidinium Thiocyanate Supplemented With Tris(2-Carboxyethyl)Phosphine.
Not available
S-EPMC8097045 | biostudies-literature
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