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GenomicsRibosome profiling of SMN-primed ribosomes and active ribosome profiling from brains of a mouse model of severe SMA
Ribosome profiling of SMN-primed ribosomes and active ribosome profiling from brains of a mouse model of severe SMA
PRJNA645135 | ENA
TranscriptomicsSelective ribosome profiling reveals the co-translational chaperone action of trigger factor in vivo
As nascent polypeptides exit ribosomes, they are engaged by a series of processing, targeting and folding factors. Here we present a selective ribosome profiling strategy that enables global monitoring of when these factors engage polypeptides in the complex cellular environment. Studies of the Esch...
ORGANISM(S): Escherichia coli 
2011-12-09 | E-GEOD-33671 | biostudies-arrayexpress
ProteomicsTranslational specialization in pluripotency by RBPMS poises future lineage-decisions
Labeling active ribosome associated proteins by L-Azidohomoalanine (AHA)- pulldown. Humane embryonic stem cells were labeled via AHA in methionine-free medium for 40 min. AHA labeled nascent proteins together with ribosomes and associated proteins were isolated with beads. Samples were divided into ...
ORGANISM(S): Homo sapiens (Human) 
2023-10-26 | PXD032046 | Pride
Pluripotency Active-ribosome associated proteins Exit of pluripotency Rbpms 
TranscriptomicsRibosome profiling of chondrocytic cell line SW1353 following exposure to IL-1B
Osteoarthritis is a chronic disease characterised by the loss of articular cartilage in synovial joints through a process of extracellular matrix destruction that is strongly associated with inflammatory stimuli. Chondrocytes undergo changes to their protein translation capacity during osteoarthriti...
ORGANISM(S): Homo sapiens 
2019-07-12 | E-MTAB-7466 | biostudies-arrayexpress
TranscriptomicsGenome-wide translational changes induced by the prion [PSI+]
Prions are infectious proteins that can adopt a structural conformation different from that of the normal protein. This change of conformation is then propagated among other molecules of the same protein. Prions are associated with neurodegenerative diseases in mammals, but are also found in fungi (...
ORGANISM(S): Saccharomyces cerevisiae 
2014-07-18 | E-GEOD-41590 | biostudies-arrayexpress
OtherRibosome profiling of SMN-primed ribosomes and active ribosome profiling from brains of a mouse model of severe SMA
Spinal muscular atrophy (SMA) is the most common genetic cause of infant mortality, with an incidence of around 1 in 6,000-10,000 live births. SMA is caused by low levels of full-length survival of motor neuron protein (SMN). We demonstrate that SMN binds to ribosomes and that this interaction is ti...
ORGANISM(S): Mus musculus 
2020-08-11 | GSE154106 | GEO
ProteomicsRPL40 methyltransferase discovery and characterisation
The eukaryotic ribosome is highly modified by protein methylation, yet many of the responsible methyltransferases remain unknown. Here we have identified SMYD5 as a ribosomal protein methyltransferase that catalyses trimethylation of RPL40 at lysine 22. Through a systematic mass spectrometry-based a...
ORGANISM(S): Homo Sapiens 
2025-04-16 | PXD056489 | panorama
TranscriptomicsFunctional characterization of C. elegans Y-box binding proteins reveals tissue-specific functions and a critical role in the formation of polysomes (RNA-Seq)
We present a basic characterization of the function of Y-box binding proteins in C. elegans. Besides playing an important role for fertility in the germline (all four CEY proteins), we found that the presence of CEY-1 and CEY-4 is essential for the assembly of larger polysomes in the soma. We theref...
ORGANISM(S): Caenorhabditis elegans 
2015-01-08 | E-GEOD-62858 | biostudies-arrayexpress
TranscriptomicsRibosome Profiling of Mouse Embryonic Stem Cells Reveals the Complexity of Mammalian Proteomes
The ability to sequence genomes has far outstripped approaches for deciphering the information they encode. Here we present a suite of techniques, based on ribosome profiling (the deep-sequencing of ribosome-protected mRNA fragments), to provide genome-wide maps of protein synthesis as well as a p...
ORGANISM(S): Mus musculus 
2011-11-03 | E-GEOD-30839 | biostudies-arrayexpress
TranscriptomicsTranscriptome-wide Regulation of Splicing and mRNA Localization by Muscleblind Proteins
The Muscleblind-like (Mbnl) family of RNA-binding proteins plays important roles in muscle and eye development and in Myotonic Dystrophy (DM), where expanded CUG or CCUG repeats functionally deplete Mbnl proteins. We identified transcriptome-wide functional and biophysical targets of Mbnl proteins i...
ORGANISM(S): Drosophila melanogaster 
2012-08-17 | E-GEOD-39911 | biostudies-arrayexpress
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