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MetabolomicsPRODH regultes naive pluripotency by influencing mitochondrial oxidative phosphorylation (Targeted metabolomics)

Naïve human embryonic stem cells (hESCs) that resemble the pre-implantation epiblasts are fueled by a combination of aerobic glycolysis and oxidative phosphorylation, but their mitochondrial regulators are poorly understood. Here we report that, proline dehydrogenase (PRODH), a mitochondria-local...

2024-02-12 | MTBLS7832 | MetaboLights
MetabolomicsPRODH regultes naive pluripotency by influencing mitochondrial oxidative phosphorylation (Untargeted metabolomics)

Naïve human embryonic stem cells (hESCs) that resemble the pre-implantation epiblasts are fueled by a combination of aerobic glycolysis and oxidative phosphorylation, but their mitochondrial regulators are poorly understood. Here we report that, proline dehydrogenase (PRODH), a mitochondria-local...

2024-02-12 | MTBLS7840 | MetaboLights
MetabolomicsDissection of two routes to naïve pluripotency using different kinase inhibitors
Embryonic stem cells (ESCs) can be maintained in the naïve state through inhibition of Mek1/2 and Gsk3 (2i). A relevant effect of 2i is the inhibition of Cdk8/19, which are negative regulators of the Mediator complex, responsible for the activity of enhancers. Inhibition of Cdk8/19 (Cdk8/19i) stimul...
2021-02-10 | MTBLS301 | MetaboLights
MetabolomicsStirred suspension bioreactors maintain naïve pluripotency of human pluripotent stem cells (hPSCs)
Not available
ORGANISM(S): Homo sapiens 
ST001377 | MetabolomicsWorkbench
TranscriptomicsZscan10 is dispensable for maintenance of pluripotency in mouse embryonic stem cells
Zinc finger and SCAN domain-containing 10 (Zscan10, also known as Zfp206) encodes a transcription factor that has been reported to be involved in the maintenance of pluripotency in mouse embryonic stem (ES) cells. Here we generated inducible knockout ES cells for Zscan10 using the Cre-loxP system an...
ORGANISM(S): Mus musculus 
2015-12-20 | E-GEOD-76143 | biostudies-arrayexpress
ProteomicsSecreted footprint of pluripotency in REBL.PAT IPSCs
With a view to developing a much-needed non-invasive method for monitoring the healthy pluripotent state of human stem cells in culture, we undertook proteomic analysis of the waste medium from cultured induced (Rebl.PAT) human pluripotent stem cells (hPSCs). Cells were grown in E8 medium to maintai...
ORGANISM(S): Homo sapiens (Human) 
2024-03-26 | PXD045347 | Pride
Pluripotency Ipsc Stem cells 
ProteomicsSecreted footprint of pluripotency in Man-13 HeSCs
With a view to developing a much-needed non-invasive method for monitoring the healthy pluripotent state of human stem cells in culture, we undertook proteomic analysis of the waste medium from cultured induced (Rebl.PAT) human pluripotent stem cells (hPSCs). Cells were grown in E8 medium to maintai...
ORGANISM(S): Homo sapiens (Human) 
2024-03-26 | PXD045525 | Pride
Pluripotency Ipsc Stem cells 
ProteomicsA mass spectrometry survey of chromatin-associated proteins in pluripotency and early lineage commitment
Pluripotent Embryonic Stem Cells (ESCs) can be captured in vitro in different states, ranging from unrestricted ‘naïve’ to more developmentally constrained ‘primed’ pluripotency. Complexes involved in epigenetic regulation and key transcription factors have been shown to be involved in specifying th...
ORGANISM(S): Mus musculus (Mouse) 
2019-07-04 | PXD011782 | Pride
Differentiation Naïve pluripotency Lc-ms Primed pluripotency Mouse Chromatin 
Metabolomics1H NMR metabolite monitoring during the differentiation of human induced pluripotent stem cells provides new insights into the molecular events that regulate embryonic chondrogenesis
The integration of cell metabolism with signalling pathways, transcription factor networks and epigenetic mediators is critical in coordinating molecular and cellular events during embryogenesis. Induced pluripotent stem cells (IPSCs) are an established model for embryogenesis, germ layer specificat...
2023-05-24 | MTBLS4854 | MetaboLights
ProteomicsThe translational landscape of naïve and primed state pluripotency
Translational control plays a central role in regulation of gene expression and can lead to significant divergence between mRNA- and protein-abundance. The translational landscape of early mammalian development and its impact on cellular proteome, however, remains largely un-explored. Here we used g...
ORGANISM(S): Mus musculus (Mouse) 
2020-04-03 | PXD014528 | Pride
Ribosome profiling Proteome Translation Embryonic stem cells Ground state pluripotency 
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