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This study characterizes the gene expression changes during the differentiation of the human embryonic stem cell (hESC) line Man13 into hypertrophic-like cartilage pellets. Samples include five key stages: pluripotent cells (TeSR™-E8™), induced mesenchymal stromal cells (iMSCs), and three-dimensiona...
ORGANISM(S): Homo sapiens 
PBMCs from MED patients carrying the MATN3 V194D mutation and unaffected controls were reprogrammed into hiPSCs. Samples were differentiated into Day 21 cartilage pellets via an iMSC intermediate.
ORGANISM(S): Homo sapiens 
PBMCs from MED patients carrying the MATN3 T195K mutation and unaffected controls were reprogrammed into hiPSCs using Sendai virus. Data includes three differentiation stages: pluripotent hiPSCs, iMSCs, and Day 21 cartilage pellets. Sequencing was performed at the University Genomics Core Facility.
ORGANISM(S): Homo sapiens 
RNA-seq of control and MATN3 V194D mutant cartilage pellets. The MATN3 V194D mutation was introduced using CRISPR-Cas9 in pluripotent cells before differentiation through an iMSC intermediate to Day 21 cartilage pellets.
ORGANISM(S): Homo sapiens 
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