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ProteomicsRewiring the metabolic network to increase docosahexaenoic acid productivity in Crypthecodinium cohnii by fermentation supernatant-based adaptive laboratory evolution
To overcome the inhibition caused by the fermentation supernatant in the late fermentation stage of docosahexaenoic acid (DHA)-producing Crypthecodinium cohnii, fermentation supernatant-based adaptive laboratory evolution (FS-ALE) was conducted. The cell growth and DHA productivity of the evolved st...
ORGANISM(S): Crypthecodinium Cohnii 
2021-11-27 | PXD030028 |
ProteomicsSimultaneous Optimization of Multi-lignocellulosic-sugar Catabolism via Systematic Laboratory Evolution in Dynamic Conditions
The development of microbial bioproduction is challenged by the complexity and variability of industrial feedstocks. This study aimed to systematically and simultaneously optimize catabolism of multiple sugars present in common renewable lignocellulose by adaptive laboratory evolution (ALE) using dy...
ORGANISM(S): Pseudomonas putida KT2440 
2026-06-29 | PXD055215 | Pride
Pseudomonas putida Adaptive laboratory evolution (ale) Proteomics Lignocellulose 
ProteomicsAutotrophic adaptive laboratory evolution of the acetogen Clostridium autoethanogenum delivers the gas-fermenting strain LAbrini with superior growth, products, and robustness
Microbes able to convert gaseous one-carbon (C1) waste feedstocks are increasingly important to transition to the sustainable production of renewable chemicals and fuels. Acetogens are interesting biocatalysts since gas fermentation using Clostridium autoethanogenum has been commercialised. However,...
ORGANISM(S): Clostridium autoethanogenum 
2024-06-12 | PXD047330 | Pride
Chemostat Proteomics Adaptive laboratory evolution Gas fermentation Reverse genetic engineering Whole-genome sequencing 
ProteomicsAdaptive Laboratory Evolution of E. coli towards usage of fluoroindole
Although fluorine is abundant in the earth’s crust, it is scarcely found in biomolecules. Adaptive laboratory evolution (ALE) experiments were conducted to introduce organofluorine into living microorganisms. By cultivating Escherichia coli with fluorinated indole analogs, microbial cells evolved th...
ORGANISM(S): Escherichia coli 
2024-01-08 | PXD048225 | Pride
7-fluoroindole E. coli Adaptive laboratory evolution (ale) 6-fluoroindole Xenonutrient Nanolc-ms/ms 
TranscriptomicsRNAseq analysis of global gene expression patterns in Corynebacterium glutamicum Adaptive Laboratory Evolution (ALE) strains GluA T5 and GluA T7 with accelerated growth and glutarate production.
The Adaptive Laboratory Evolution (ALE) experiment allowed to select Corynebacterium glutamicum strain GluA T5, which grew faster and produced more glutarate than the parent strain GluA T0, and strain GluA T7 that grew as fast as GluA T5, but produced more 5AVA than GluA T5. To explore differences i...
ORGANISM(S): Corynebacterium glutamicum ATCC 13032 
2021-01-31 | E-MTAB-10025 | biostudies-arrayexpress
GenomicsAdaptive laboratory evolution of mutS Knock-out E. coli
Adaptive laboratory evolution of mutS Knock-out E. coli
PRJEB32586 | ENA
GenomicsAdaptive laboratory evolution of a genome-reduced Escherichia coli
Adaptive laboratory evolution of a genome-reduced Escherichia coli
PRJEB21199 | ENA
MetabolomicsModel-guided development of evolutionarily stable yeast chassis for dicarboxylic acids production

Chassis strain suitable for producing multiple compounds is a central concept in synthetic biology. Design of a chassis using computational, first-principle, models is particularly attractive due to the predictability and control it offers, including against phenotype reversal due to adaptive mut...

2021-07-19 | MTBLS2007 | MetaboLights
ProteomicsAdaptive laboratory evolution recruits the promiscuity of succinate semialdehyde dehydrogenase to repair different metabolic deficiencies
Metabolism is fundamental to life, all life activities are sustained through metabolism. Metabolic engineering, while modifying host endogenous metabolic network or introducing heterologous pathway for biotechnological applications, often results in unfitness and suboptimal characteristics, because ...
ORGANISM(S): Escherichia coli 
2024-09-10 | PXD048035 | Pride
E.coli 
ModelsKonstantinidis2021 - Persistence of mutants in a mutualistic microbial co-culture
This agent-based model is based on an adaptive laboratory evolution (ALE) experiment scenario of two mutually cross feeding strains of bacteria and yeast. The bacterial strain secretes vitamins for which the yeast strain is auxotrophic and the yeast strain secrets amino acids for which the bacterial...
2022-08-01 | MODEL2106100001 | BioModels
Manuscript Accepted 
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