Affinity isolation

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Proteomics (1979)
Unknown (956)
Transcriptomics (621)
Genomics (195)
Metabolomics (21)
Other (5)
Multiomics (3)
Methylation profiling (2)

Organisms

Homo sapiens (337)
Mus musculus (259)
Saccharomyces cerevisiae (34)
Caenorhabditis elegans (25)
Arabidopsis thaliana (21)
Drosophila melanogaster (14)
Rattus norvegicus (10)
Danio rerio (10)
Escherichia coli (8)
Sinorhizobium meliloti (7)
Bos taurus (5)
Bacillus subtilis subsp. subtilis str. 168 (4)
Zea mays (4)
Oncorhynchus mykiss (4)
Bacillus subtilis (3)
Gallus gallus (3)
Escherichia coli str. K-12 substr. MG1655 (3)
Cryptococcus neoformans (3)
Macaca mulatta (2)
Corynebacterium glutamicum (2)
Chlorocebus sabaeus (2)
Staphylococcus aureus (2)
Escherichia coli O127:H6 str. E2348/69 (1)
Saccharomyces cerevisiae x Saccharomyces paradoxus (1)
Cercocebus atys (1)
Porphyromonas gingivalis ATCC 33277 (1)
Tetrahymena thermophila (1)
Euglena gracilis (1)
Populus trichocarpa x Populus deltoides (1)
Lupinus angustifolius (1)

Organisms

Mus musculus (35)
Homo sapiens (25)
Drosophila melanogaster (6)
Danio rerio (4)
Arabidopsis thaliana (4)
Saccharomyces cerevisiae (2)
Corynebacterium glutamicum ATCC 13032 (1)
Mammaliicoccus sciuri (1)
Vicugna pacos (1)
Yersinia pestis (1)
Cryptococcus neoformans (1)
Pseudomonas aeruginosa (1)
Pseudomonas putida (1)

Repository

pride (1740)
biostudies-arrayexpress (660)
MassIVE (168)
ENA (104)
geo (54)
jPOST (48)
MetaboLights (20)
iProX (12)
EGA (5)
PanoramaPublic (4)
PAXDB (4)
PeptideAtlas (3)
GNPS (1)

Tissue

Cell culture (561)
Epithelial cell (149)
Permanent cell line cell (106)
Brain (72)
Plant cell (47)
Blood (44)
B cell (43)
Fibroblast (42)
Cell suspension culture (40)
Kidney (39)
Embryonic stem cell (36)
Leaf (36)
Liver (33)
Blood cell (32)
Blood plasma (31)
HeLa cell (31)
Lung (31)
T cell (28)
Heart (24)
Seedling (23)
Spleen (19)
Early embryonic cell (18)
Embryo (18)
Primary cell (18)
Macrophage (16)
Skin (15)
Whole body (15)
HEK-293T cell (13)
Colon (13)
Others (3)

Disease

Disease free (49)
Breast cancer (40)
Acute leukemia (25)
Colon cancer (23)
Cervix carcinoma (22)
Melanoma (12)
Brain cancer (12)
Alzheimer's disease (12)
Amyotrophic lateral sclerosis (12)
Influenza (10)
Plasmodium falciparum malaria (9)
Trypanosomiasis (9)
Non-small cell lung carcinoma (8)
Lung cancer (8)
Neuroblastoma (8)
Type 2 diabetes mellitus (8)
Lymphoma (7)
Brain glioblastoma multiforme (6)
COVID-19 (6)
Huntington Disease (5)
Lung adenocarcinoma (5)
Multiple myeloma (5)
Skin cancer (4)
Benign prostatic hyperplasia (2)
Normal (2)
Fatty liver disease (1)
Vitamin B12 deficiency (1)
MAS (1)
Acute myelomonocytic leukaemia (1)
Cirrhosis (1)

Technology Type

Mass Spectrometry (1740)
Affinity purification coupled with mass spectrometry proteomics (1147)
Shotgun proteomics (641)
Bottom-up proteomics (226)
Data-dependent acquisition (184)
Gel-based experiment (115)
Data-independent acquisition (59)
Chemical cross-linking coupled with mass spectrometry proteomics (56)
Top-down proteomics (42)
Affinity proteomics (39)
SRM/MRM (35)
SWATH MS (32)
Mass spectrometry (16)
All-ion fragmentation (8)
Immunopeptidomics (7)
DiaPASEF (6)
Mass spectrometry assay (4)
Mass spectrometry imaging (3)
MSE (3)
Glycoproteomics (2)
HDMSE (2)
Illumina (1)
Illumina Genome Analyzer II (1)
Olink affinity proteomics (1)
PAcIFIC (1)
Proteogenomics (1)
RNA mass spectrometry (1)

Instrument Platform

Illumina NovaSeq 6000 (36)
NextSeq 500 (18)
Illumina HiSeq 2500 (17)
Illumina HiSeq 4000 (16)
Illumina HiSeq 2000 (11)
Illumina HiSeq 1500 (8)
NextSeq 550 (8)
Illumina NovaSeq X (6)
Illumina MiSeq (4)
Illumina Genome Analyzer IIx (2)
Illumina Genome Analyzer II (2)
Flow Cytometer (2)
Affymetrix (2)
NA (2)
NextSeq 2000 (2)
-- (1)
Thermomixer (1)
Dell T8310 (1)
Agilent 2100 bioanalyzer (1)
NanoDrop ND-1000 (1)
QIAcube (Qiagen) (1)
EpMotion 5075 automated pipetting system (Eppendorf, Hamburg, Germany) (1)
Retsch MM400 (1)
Agilent Drosophila Gene Expression Microarray, 4x44K arrays; Agilent Microarray Hybridization Oven (G2545A) (1)
Agilent Scanner C (1)
In Silico Molecular Cloning Program (1)
GeneChip® Scanner 3000 7G (1)
Affymetrix GeneChip Scanner 3000 7G (1)
ScanArray 4000 [PerkinElmer] (1)
418 [Affymetrix] (1)

Publication Date

2023 (298)
2025 (282)
2022 (266)
2024 (265)
2021 (226)
2026 (200)
2020 (185)
2019 (146)
2016 (114)
2018 (111)
2015 (108)
2013 (103)
2017 (73)
2014 (71)
2012 (61)
2010 (59)
2011 (40)
2008 (35)
2009 (20)
2007 (18)
2005 (8)
2006 (3)
2004 (2)

UNIPROT ID/AC

E0CYZ8_MOUSE (3)
TCL1A_MOUSE (3)
A14EL_MOUSE (3)
TSX_MOUSE (3)
ATG12_MOUSE (3)
RPAB2_MOUSE (3)
DPPA4_MOUSE (3)
S1PBP_MOUSE (3)
ZN287_MOUSE (3)
RPP25_MOUSE (3)
PALM3_MOUSE (3)
CBPC3_MOUSE (3)
MTF1_MOUSE (3)
CB076_MOUSE (3)
TCAL8_MOUSE (3)
DPA5A_MOUSE (3)
BICRL_MOUSE (3)
RTEL1_MOUSE (3)
AEBP2_MOUSE (3)
F10C1_MOUSE (3)
SKA2_MOUSE (3)
RN138_MOUSE (3)
TYDP2_MOUSE (3)
AF10_MOUSE (3)
RBIS_MOUSE (3)
PCGF5_MOUSE (3)
GEMI_MOUSE (3)
RNF12_MOUSE (3)
ALKB1_MOUSE (3)
SKA1_MOUSE (3)

ENSEMBL ID

ENSG00000170477 (3)

CHEBI ID

CHEBI:15843 (5)
CHEBI:28125 (4)
CHEBI:28364 (4)
CHEBI:19138 (4)
CHEBI:28728 (4)
CHEBI:15551 (4)
CHEBI:64017 (4)
CHEBI:72639 (4)
CHEBI:16335 (4)
CHEBI:16027 (4)
CHEBI:34151 (3)
CHEBI:88345 (3)
CHEBI:72783 (3)
CHEBI:72606 (3)
CHEBI:72605 (3)
CHEBI:15555 (3)
CHEBI:17351 (3)
CHEBI:72651 (3)
CHEBI:16543 (3)
CHEBI:17203 (3)
CHEBI:17964 (3)
CHEBI:17929 (3)
CHEBI:16020 (3)
CHEBI:17345 (3)
CHEBI:16695 (3)
CHEBI:138295 (2)
CHEBI:88457 (2)
CHEBI:81565 (2)
CHEBI:138645 (2)
CHEBI:138648 (2)

Metabolite Name

AA (4)
DHA (4)
TXB2 (4)
EPA (4)
9-HODE (4)
12-HETE (4)
13-HODE (4)
15-HETE (4)
Tetranor-12-HETE (3)
12-HHTrE (3)
12-HEPE (3)
8-HDoHE (3)
11-HETE (3)
PGD2 (3)
PGE2 (3)
12-OxoETE (3)
12(13)-EpOME (3)
Resolvin D3 (2)
15d-PGJ2 (2)
8(9)-EET (2)
5,6-DiHETE (2)
PGI2 (2)
Gln-Tyr (2)
Trans,trans-Muconic acid (2)
14,15-DHET (2)
Met-Phe (2)
Asn-Trp (2)
Resolvin D1 (2)
1-methylpseudouridine (2)
3-Aminoisobutyric acid (2)

First Public Date

2014 (14)
2025 (12)
2020 (12)
2026 (11)
2024 (10)
2022 (9)
2017 (9)
2023 (6)
2021 (6)
2019 (5)
2018 (5)
2016 (3)
2015 (1)
2013 (1)

Classification

bacteria (3)
fungi (1)

Modification

2x(15)N labeled L-lysine (5)
4x(15)N labeled L-arginine (3)
4'-dihydroxyphenylalanine (1)
6-dioxaoctanediamine derivatized cysteine (1)
-17.026549 (1)
42.010565 (1)
15.994915 (1)
N6 (1)

Study type

Transcription profiling by array (310)
RNA-seq of coding RNA (123)
ChIP-seq (64)
RNA-seq of coding RNA from single cells (30)
Transcription profiling by tiling array (21)
Other (19)
Methylation profiling by array (9)
RNA-seq of non coding RNA (9)
ChIP-chip by tiling array (8)
Unknown experiment type (6)
ChIP-chip by array (6)
Ribo-seq (6)
MicroRNA profiling by array (6)
Methylation profiling by high throughput sequencing (6)
Human - High-throughput sequencing (5)
Human - Single-cell sequencing (3)
Comparative genomic hybridization by array (3)
RIP-seq (3)
RNA-seq of total RNA (3)
High-throughput sequencing (2)
Cell line - High-throughput sequencing (2)
Transcription profiling by RT-PCR (2)
Transcription profiling by MPSS (1)
Transcription profiling by SAGE (1)
RNA-seq of non coding RNA from single cells (1)
Differential (1)
RIP-chip by array (1)
Animal - Single-cell sequencing (1)
Human - One-color microarray (1)
Animal - High-throughput sequencing (1)

Release Date

2015 (128)
2013 (119)
2022 (113)
2016 (104)
2014 (102)
2021 (95)
2023 (89)
2024 (86)
2012 (85)
2020 (84)
2025 (80)
2010 (80)
2019 (72)
2011 (69)
2018 (66)
2008 (46)
2017 (44)
2009 (43)
2007 (26)
2026 (22)
2005 (18)
2006 (8)
2004 (8)
2002 (6)
2001 (6)
1998 (5)
2000 (3)
1999 (3)
1997 (3)
2003 (1)

Lab affiliation

Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, MA 02215, USA., Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA (27)
Proteomics program, Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, 2200 Copenhagen, Denmark (16)
Basic Immune System of Plants / Protein Mass Spectrometry Max Planck Institute for Plant Breeding Research Carl-von-Linne-Weg 10, 50829 Cologne, Germany (15)
Centre for Genomic Regulation (13)
The Institute of Cancer Research (12)
University of Oxford (10)
Ghent University, Department of Plant Biotechnology and Bioinformatics, Technologiepark 71, 9052 Ghent, Belgium VIB Center for Plant Systems Biology, Technologiepark 71, 9052 Ghent, Belgium (8)
Department of Proteomics and Signal Transduction, Max-Planck-Institute of Biochemistry, Munich, Germany (8)
Department Biochemistry of Plant Interactions, Leibniz Institute of Plant Biochemistry, 06120 Halle (Saale), Germany (7)
Indiana University School of Medicine (7)
Leibniz Institute of Plant Biochemistry, Department Biochemistry of Plant Interactions, Weinberg 3, 06120 Halle (Saale), Germany (5)
Department of Experimental Radiation Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA (5)
University of Washington (5)
UCSF (5)
Chair of Biochemistry II, Theodor Boveri-Institute, Biocenter, University of Würzburg, Würzburg, Germany (5)
Technical University of Munich, School of Medicine, Institute of Virology, 81675 Munich, Germany (5)
Cambridge Institute for Medical Research, University of Cambridge, Hills Road, Cambridge, CB2 0XY, UK (5)
Analytics Core Facility Essen (ACE), Chemische Biologie, Universität Duisburg-Essen, ZMB, Germany (5)
Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, Bunkyo-ku, Tokyo 113-8656, Japan. (4)
Faculty of Biology, Technion - Israel Institute of Technology (4)
Center of Molecular and Cellular Oncology, Yale University (4)
Institute of Physiology II Universität Freiburg Germany (4)
The Rockefeller University (4)
CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, 1090 Vienna, Austria (4)
The Institute of Cancer Research, London (4)
IMol Polish Academy of Sciences, Warsaw, Poland (4)
Wellcome Trust Sanger Institute (4)
The University of Melbourne (4)
Molecular Leukemogenesis Group, German Cancer Research Center, Heidelberg, Germany Heidelberg University, Medical Faculty, Heidelberg, Germany, 6Internal Medicine V, Heidelberg University Hospital, Heidelberg, Germany (3)
Medical University of Vienna, Max Perutz Labs, Dr. Bohr-Gasse 9, 1030 Vienna (3)

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LipidCreator workbench to probe the lipidomic landscape - Platelet isolation and stimulation, Targeted Phospho and Glycerolipid Profiling

Here we present LipidCreator, a software that fully supports targeted lipidomics assay development. LipidCreator offers a comprehensive framework to compute MS/MS fragment masses for over 60 lipid classes. LipidCreator provides all functionalities needed to define fragments, manage stable isotope...

2020-04-15 | MTBLS1369 | MetaboLights

LipidCreator workbench to probe the lipidomic landscape - Platelet isolation and stimulation, targeted lipid mediator profiling

2020-04-15 | MTBLS1381 | MetaboLights

LipidCreator workbench to probe the lipidomic landscape - Platelet isolation and stimulation - DIA lipid mediator validation

2020-04-15 | MTBLS1382 | MetaboLights

Benchmark study of different chromatin isolation methods using the E2Fa transcription factor

Genome-wide identification of transcription factor (TF) binding sites is pivotal to our understanding of gene expression regulation. Although much progress has been made in the determination of potential binding regions of proteins by chromatin immunoprecipitation (ChIP), this method has some inhere...

ORGANISM(S): Arabidopsis thaliana

2014-02-04 | E-GEOD-53422 | biostudies-arrayexpress

RNA affinity isolation with TAP-Pum and controls

Biochemical purification: Five grams of adult flies or 2.5 g of embryos were used in each affinity purification. Flies or embryos were suspended in 15 ml of buffer B (buffer A plus 1.5 mM dithiothreitol [DTT], 1 mM phenylmethylsulfonylfluoride [PMSF], 0.5 5g/ml leupeptin, 0.8 5g/ml pepstatin, 20 U/m...

ORGANISM(S): Drosophila melanogaster

2006-02-28 | E-GEOD-3582 | biostudies-arrayexpress

Identification of Mitogen-Activated Protein Kinase Phosphatase-1 (MKP-1) protein partners using Tandem Affinity Purification and Mass Spectrometry

According to the World Health Organization Report, depressive disorders affect about 10% of the population. The molecular mechanism of the pathogenesis of depression is still not well understood. The new findings point to phosphatases as potential targets for effective depression therapy. The aim of...

ORGANISM(S): Rattus Norvegicus (ncbitaxon:10116)

2023-01-19 | MSV000091093 | MassIVE

Rapid isolation of extracellular vesicles from cell culture and biological fluids using a synthetic peptide with specific affinity for heat shock proteins

Background: Exosomes and extracellular vesicles (EVs) are increasingly recognized as important sources of biomarkers for disease study and diagnosis. Results: A synthetic peptide, Vn96, allows for capture of EVs from biological fluids using basic laboratory equipment. Conclusion: The Vn96-captured E...

ORGANISM(S): Homo sapiens

2014-06-14 | E-GEOD-58464 | biostudies-arrayexpress

Ago-APP: a GW peptide-based Argonaute affinity capture strategy

During microRNA (miRNA)-guided gene silencing, Argonaute (Ago) proteins interact with a member of the TNRC6/GW protein family. Here we used a short GW protein-derived peptide fused to GST and demonstrate that it binds to Ago proteins with high affinity. This allows for the simultaneous isolation of ...

ORGANISM(S): Arabidopsis thaliana

2015-09-08 | E-GEOD-70553 | biostudies-arrayexpress

INTACT method for gene expression and chromatin profiling of individual cell types within a tissue

We used in vivo biotin labeling of a nuclear envelope protein in individual cell types followed by affinity isolation of labeled nuclei to measure gene expression and chromatin features of the hair and non-hair cell types of the Arabidopsis root epidermis. Keywords: Chromatin affinity-purification ...

ORGANISM(S): Arabidopsis thaliana

2010-06-13 | E-GEOD-19654 | biostudies-arrayexpress

Affinity-Based Magnetic Nanoparticle Development for Cancer Stem Cell Isolation.

Not available

S-EPMC10818538 | biostudies-literature

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