Sort   by:  
 Page size 
GenomicsThe Mediator complex subunit MED23 couples H2B mono-ubiquitination to transcriptional control and cell fate determination
The Mediator complex orchestrates multiple transcription factors with the Pol II apparatus for precise transcriptional control. However, its interplay with the surrounding chromatin remains poorly understood. Here, we analyze differential histone modifications between WT and MED23-/- (KO) cells and ...
ORGANISM(S): Mus musculus 
2015-09-16 | E-GEOD-59518 | biostudies-arrayexpress
ProteomicsLight-controlled affinity purification of protein complexes
Methods to affinity purify proteins are widely used in protein research. One important application is to identify interacting proteins of an affinity-purified protein of interest (POI) by mass spectrometry. Here, we developed an optogenetics-derived and light-controlled affinity purification method ...
ORGANISM(S): Homo sapiens (Human) 
2019-03-18 | PXD012156 | Pride
Affinity purification Mass spectrometry Optogenetics Protein-protein interaction Phytochrome Zap70 
ProteomicsDifferential centrifugation coupled to affinity purification-mass spectrometry of mcherry-ATG8E
Autophagosomes are double-membraned vesicles that traffic harmful or unwanted cellular macromolecules to the vacuole for recycling. Although autophagosome biogenesis has been studied extensively, autophagosome maturation, i.e., delivery and fusion with the vacuole, remains largely unknown in plants....
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-10-18 | PXD031787 | Pride
Arabidopsis Lc-msms Autophagosomes Affinity purification 
GenomicsBRG1 recruitment by transcription factors MITF and SOX10 defines a specific configuration of regulatory elements in the melanocyte lineage (ChIP-seq)
Microphthalmia-associated transcription factor (MITF) is the master regulator of the melanocyte lineage. By tandem affinity purification and mass spectrometry, we present a comprehensive characterisation of the MITF interactome comprising multiple novel cofactors involved in transcription, DNA repli...
ORGANISM(S): Homo sapiens 
2015-04-02 | E-GEOD-61965 | biostudies-arrayexpress
ProteomicsA novel inducible retroviral expression system for StrepHA-tandem affinity purification mass-spectrometry-based proteomics
Tandem-affinity purification mass spectrometry using the streptavidin-hemagglutinin (SH)-tag has been successfully employed to map signaling networks in several large-scale studies. However its application has so far been restricted to the small number of Flp/FRT-recombination competent cell lines....
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2016-01-05 | PXD002855 | Pride
Tandem affinity purification-mass spectrometry Protein-protein interaction Streptavidin-hemagglutinin-tag 
TranscriptomicsBRG1 recruitment by transcription factors MITF and SOX10 defines a specific configuration of regulatory elements in the melanocyte lineage (RNA-seq)
Microphthalmia-associated transcription factor (MITF) is the master regulator of the melanocyte lineage. By tandem affinity purification and mass spectrometry, we present a comprehensive characterisation of the MITF interactome comprising multiple novel cofactors involved in transcription, DNA repli...
ORGANISM(S): Homo sapiens 
2015-04-02 | E-GEOD-61966 | biostudies-arrayexpress
ProteomicsAPPLE-MS: A modular affinity purification-mass spectrometry method assisted by PafA- mediated proximity labeling
While affinity purification-mass spectrometry (AP-MS) has significantly advanced protein-protein interaction (PPI) studies, its limitations in detecting weak, transient, and membrane-associated interactions remain. To address these challenges, we introduced an innovative proteomic method termed Affi...
ORGANISM(S): Homo sapiens (Human) Rattus norvegicus (Rat) 
2025-10-27 | PXD063145 | Pride
Ap-ms Proximity labeling Protein-protein interaction Interactome 
ProteomicsTandem affinity purification of histones, coupled to mass spectrometry, identifies new sites of post-translational modification and associated proteins in Saccharomyces cerevisiae
Our results can be considered as a starting point to gain understanding of how nucleosome modification and composition affect basic cellular processes in yeast.
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2016-01-28 | PXD002671 | Pride
Chromatin Yeast Histones Tandem affinity purification Proteomics 
ProteomicsIdentification of SCGB3A1-interacting proteins by pull-down coupled with mass spectrometry
Affinity purification coupled with mass spectrometry was performed to identify proteins interacting with SCGB3A1. The identified candidate proteins were analyzed to determine potential binding partners involved in SCGB3A1-mediated biological functions.
ORGANISM(S): Mus musculus (Mouse) 
2026-09-03 | PXD083435 | Pride
Pull-down assay Slc44a2 Scgb3a1 Protein-protein interaction Affinity purification mass spectrometry 
TranscriptomicsMS2-affinity purification coupled with RNA sequencing (MAPS) reveals RyhB sRNA targetome
Despite the overwhelming information about sRNAs, one of the biggest challenges in the sRNA field is characterizing sRNA targetomes. Thus, we develop a novel method to identify RNAs that interact with a specific sRNA, regardless of the type of regulation (positive or negative) or targets (mRNA, tRNA...
ORGANISM(S): Escherichia coli K-12 
2015-04-16 | E-GEOD-66519 | biostudies-arrayexpress
Sort   by:  
 Page size