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TranscriptomicsRNAi knock-down of CAF1-CCR4-NOT1 deadenylase complex or AGO1 in D. melanogaster S2 cells
miRNAs silence gene expression by repressing translation and/or by promoting mRNA decay. AGO1 is a key protein required for miRNA-mediated gene silencing. In animal cells, mRNA degradation of partially complementary miRNA targets occurs via deadenylation by the CAF1-CCR4-NOT1 deadenylase complex, fo...
ORGANISM(S): Drosophila melanogaster 
2008-12-03 | E-MEXP-1713 | biostudies-arrayexpress
TranscriptomicsCharacterization of AGO1-/AGO4-associated smRNAs
To fully characterize smRNAs associated with AGO1 and AGO4, we developed a two-step protocol to purify AGO/smRNA complexes from flowers, leaves, roots and seedlings with enhanced purity, and sequenced the smRNAs by IlluminaM-CM-"M-BM-^@M-BM-^Ys technology. We identified some additional miRNAs, colla...
ORGANISM(S): Arabidopsis thaliana 
2011-04-19 | E-GEOD-28591 | biostudies-arrayexpress
TranscriptomicsAGO1-dependent Small RNA in Arabidopsis Identified by High-Throughput Sequencing
The goal of this study was to identify AGO1-dependent miRNA and tasiRNA in Arabidopsis by sequencing small RNA from wild type (Col-0) and ago1-25 plants. Keywords: Small RNA Sequencing-by-synthesis technology was used to sequence small RNA from flower tissue (stages 1-12) from wild-type (Col-0) and...
ORGANISM(S): Arabidopsis thaliana 
2008-11-17 | E-GEOD-13605 | biostudies-arrayexpress
TranscriptomicsAgo1 vs Ago2-IP small RNA deep-sequencing with age in Drosophila
Drosophila miRNAs show distinct change in isoform distribution pattern with age. Some miRNAs show accumulation of the short isoforms, while other miRNAs show the accumulation of the long isoforms with age. The increase of the long isoforms of some miRNAs reflects increased 2'-O-methylated miRNA isof...
ORGANISM(S): Drosophila melanogaster 
2014-01-01 | E-GEOD-52961 | biostudies-arrayexpress
TranscriptomicsSlicer activity of Arabidopsis AGO1 in siRNA biogenesis and miRNA target decay
Aim of study The objective of these studies was to analyze the implication of slicer activity of AGO1 in the following molecular processes: loading of siRNAs and miRNAs stability of siRNAs and miRNAs formation of RDR6/SGS3-dependent secondary siRNAs decay of mRNAs targeted by miRNAs The stud...
ORGANISM(S): Arabidopsis thaliana 
2016-09-05 | E-MTAB-4529 | biostudies-arrayexpress
TranscriptomicsGenome-wide maps of AGO1-bound small RNA in Arabidopsis leaves
We report flg22 regulate the accumulation of AGO1-bound small RNA in arabidopsis. We find that a number of miRNAs are up- or down-regulated by flg22, a well-studied PAMP. Examination of AGO1-bound small RNAs with or without flg22 treatment.
ORGANISM(S): Arabidopsis thaliana 
2010-02-25 | E-GEOD-20448 | biostudies-arrayexpress
TranscriptomicsProfiling of small RNA populations in rice total extract and purified AGO1 complexes
microRNAs (miRNAs) are a class of small silencing RNAs that have regulatory roles in gene expression. miRNAs interact with Argonaute (AGO) proteins to form effector complexes that can cleave target mRNAs or repress their translation. Rice encodes four AGO1 homologs (AGO1a, AGO1b, AGO1c, and AGO1d). ...
ORGANISM(S): Oryza sativa 
2009-11-23 | E-GEOD-18250 | biostudies-arrayexpress
TranscriptomicsIdentification of small RNAs in rice AGO1 complexes and their targets
This SuperSeries is composed of the following subset Series: GSE18248: Sequencing of rice degradome GSE18250: Profiling of small RNA populations in rice total extract and purified AGO1 complexes Refer to individual Series
ORGANISM(S): Oryza sativa 
2009-11-23 | E-GEOD-18251 | biostudies-arrayexpress
TranscriptomicsRNAi knock down of four Argonaute paralogs (AGO1, AGO2, PIWI and Aubergine) and the microRNA-processing enzyme Drosha in Drosophila
RNA silencing pathways are conserved gene regulation mechanisms that lead to degradation, translational repression or transcriptional silencing of target-transcripts selected based on complementarity with small RNA molecules. The fraction of the genome regulated by these pathways has not been establ...
ORGANISM(S): Drosophila melanogaster 
2006-11-20 | E-MEXP-295 | biostudies-arrayexpress
no omics definedRNAi knock down of GW182 in Drososphila cells compared to cells with knock down of AGO1, a key effector of miRNA-mediated gene silencing
RNA silencing pathways are conserved gene regulation mechanisms that lead to degradation, translational repression or transcriptional silencing of target-transcripts selected based on complementarity with small RNA molecules. Using firefly luciferase reporters, we previously were able show that the ...
ORGANISM(S): Drosophila melanogaster 
2006-10-31 | E-MEXP-487 | biostudies-arrayexpress
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