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Helicobacter pylori strain TN2wt was suspended in Brucella broth supplemented with 5% depleted exosomes FCS. The initial optical density at 600 nm of the bacterial suspension was adjusted to 0.02 in 200 mL of Brucella broth + 0.5% depleted exosome FCS. H. pylori was grown in a 500 mL flask under mic...
ORGANISM(S): Helicobacter Pylori 
Helicobacter pylori strain ATCC51932 (Tx30a) was suspended in Brucella broth supplemented with 5% depleted exosomes FCS. The initial optical density at 600 nm of the bacterial suspension was adjusted to 0.02 in 200 mL of Brucella broth + 0.5% depleted exosome FCS. H. pylori was grown in a 500 mL fla...
ORGANISM(S): Helicobacter Pylori 
Human gastric adenocarcinoma AGS cells were infected with H. pylori Tx30a or TN2wt at a multiplicity of infection = 100 and incubated at 5% CO2,37oC for 48 h. The cells/debris were removed from the cell culture supernatant by centrifugation at 2,000 x g for 30 min at 4oC and filtered through a 0.45 ...
ORGANISM(S): Homo Sapiens (human) 
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