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ProteomicsSupporting information for tryptic EPO glycopeptide LC-MS analysis via cysteine aminoethylation
Supplementary information for a technical note describing the application of cysteine aminoethylation for the site-specific glycosylation analysis of recombinant human EPO using trypsin as sole protease.
ORGANISM(S): Cho Cells 
2020-04-21 | MSV000085318 | MassIVE
ProteomicsExtending the coverage of Lys-C/trypsin-based bottom-up proteomics using cysteine S-aminoethylation
In this study, S-aminoethylation of Cys (AE-Cys) was carried out with 2-bromoethylamine to form pseudo-Lys, which was then digested with Lys-C or trypsin to improve proteome coverage in bottom-up proteomics. A model study with bovine serum albumin showed that the C-terminal side of Cys was successfu...
ORGANISM(S): Homo Sapiens (human) Bos Taurus 
2023-09-27 | PXD045433 | JPOST Repository
UnknownQuantitative and Structural Assessment of Histone Methyllysine Analogue Engagement by Cognate Binding Proteins Reveals Affinity Decrements Relative to Those of Native Counterparts.
Not available
S-EPMC5780203 | biostudies-literature
UnknownUnusual fragmentation of derivatized cysteine-containing peptides.
Not available
S-EPMC6430700 | biostudies-literature
Proteomicsprotein coverage improvement for mouse lung tissue by using cysteine modification method
protein coverage is improved for both total protein and membrane protein by using powerful cysteine modification (S-aminoethylation) method. We successfully identify about 22% extra total protein and 19% extra membrane protein compared with conventional IAM alkylation method with ultrahigh sensitive...
ORGANISM(S): Mus Musculus (mouse) 
2021-05-26 | PXD026240 | JPOST Repository
ProteomicsCysteine capping with CAM reagent
CAM reagent was synthesized and used to uniquely convert cysteine residues, under mild conditions, into trypsin-recognsable sites. We apply this cysteine-capping methodology in the identification of missense variants in HCT-15 cell lines.2-bromoethylamine (BEA)was used as controls, for cysteine capp...
ORGANISM(S): Homo sapiens (Human) 
2025-03-13 | PXD052935 | Pride
Cam Cysteine capping Aminoethylation 
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