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Piglets cloned by somatic cell nuclear transfer (SCNT) show a high incidence of malformations and a high death rate during the perinatal period. To investigate the underlying mechanisms for abnormal development of cloned pig fetuses, we compared body weight, amniotic fluid (AF) metabolome, and place...
2020-01-08 | MTBLS666 | MetaboLights
We designed a bottom-up proteomic approach combining two complementary strategies as GeLC-MS/MS (protein samples fractionated by SDS-PAGE are analyzed by nanoLC-MS/MS after in-gel digestion) and shotgun (protein samples are directly analyzed by nanoLC-MS/MS after in-solution digestion) to give an ex...
ORGANISM(S): Gallus gallus (Chicken) 
2018-02-21 | PXD008046 | Pride
We studied the miRNA expression profile of heifers carrying male and female embryos in amniotic fluid and blood plasma at day 39 post-insemination, to determine the possible roles of miRNAs during the sex determination process.
ORGANISM(S): Bos taurus 
In this study, we carried out a comprehensive comparative proteomic and transcriptomic analysis of exosomes in amniotic fluid supernatant and cells in amniotic fluid precipitation of severe fetal hydronephrosis patients to identify candidate biomarkers with diagnostic potential. As the investigation...
ORGANISM(S): Homo sapiens (Human) 
2023-10-24 | PXD033988 | Pride
Preterm neonates are susceptible to gastrointestinal (GI) disorders such as necrotizing enterocolitis (NEC). Maternal milk, and especially colostrum, protects against NEC via growth promoting, immunomodulatory and antimicrobial factors. The fetal enteral diet, amniotic fluid (AF), contains similar b...
ORGANISM(S): Sus scrofa 
Preterm prelabour rupture of membranes beyond the 34th week of gestation (late PPROM) is frequently associated with the risk of the microbial invasion of the amniotic fluid (MIAC) and histological chorioamnionitis (HCA). Hence, we employed a Tandem Mass Tag-based approach to uncover amniotic fluid p...
ORGANISM(S): Homo sapiens (Human) 
2020-10-22 | PXD020329 | Pride
Amniotic fluid volume (AFV) is determined primarily by the rate of intramembranous (IM) transport of AF cross the amnion. Intramembranous transport is characterized as vesicular endocytotic and transcytotic processes regulated by fetal urine-derived stimulators and AF inhibitors. Our objectives were...
ORGANISM(S): Ovis aries 
2021-07-21 | PXD013135 | Pride
Primary epithelial cells isolated from fetal lungs of rat fetuses with or without lung hypoplasia induced by the administration of nitrofen to pregnant rats. Control group included epithelial cells from normal fetal lungs. Treatment with amniotic fluid stem cell derived extracellular vesicles or wit...
ORGANISM(S): Rattus norvegicus 
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