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ProteomicsIdentification of ECT2-containing protein complex
Cellular extracts from HeLa cells expressing FLAG-ECT2 were immunopurified with anti-FLAG affinity beads and eluted with FLAG peptide. The eluates were resolved on SDS/PAGE and silver stained, followed by mass spectrometric analysis.
ORGANISM(S): Homo Sapiens 
2021-06-29 | PXD026977 |
ProteomicsProteomics Characteristics Reveal the Risk of T1 Colorectal Cancer Metastasis to Lymph Nodes
The presence of lymph node metastasis (LNM) affects treatment strategy decisions in T1NxM0 colorectal cancer (CRC), but the currently used clinicopathological-based risk stratification cannot be used to accurately predict LNM. In this study, we established a model for predicting LNM and explored the...
ORGANISM(S): Homo Sapiens 
2023-04-12 | PXD041476 |
ProteomicsCyclic immonium ion of lactyllysine reveals widespread lactylation in the human proteome
Here we employed a non-enzymatic chemical labeling approach to introduce lactylation on lysine residues of model peptides and proteins. Tandem MS acquisition of lactylated peptide carriers on different MS platforms unequivocally led us to note an intense peak corresponding to the “cyclic” immonium ...
ORGANISM(S): Homo Sapiens 
2021-09-15 | PXD028488 |
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