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ProteomicsTandem affinity purification coupled with mass spectrometry (TAP-MS) to identify the proteins that interact with RAD51, RAD51 paralogs (RAD51B, RAD51C,RAD51D,XRCC2 and XRCC3), PFKP and PFKP-F649L in HeLa cells.
Tandem affinity purification coupled with mass spectrometry (TAP-MS) was used to identify the proteins that interact with RAD51,RAD51 paralogs (RAD51B, RAD51C,RAD51D,XRCC2 and XRCC3),PFKP and PFKP-F649L in HeLa cells. In addition, we also investigated the phosphorylation of RAD51.
ORGANISM(S): Homo sapiens (Human) 
2026-04-27 | PXD070095 | Pride
Rad51paralogs Rad51 Pfkp-f649l Hela cells Rad51 phosphorylation Pfkp 
ProteomicsTarget identification of dioxygenated amide derivatives interacting proteins
To identify the direct binding proteins of dioxygenated amide by chemical proteomics, we firstly evaluated the efficiency of optimized probe 6D-PP probe labelling the E. coli bacterial proteomes by in-gel fluorescence. Briefly, the E. coli bacterial lysates were incubated with 100 M probe 6D-PP und...
ORGANISM(S): Homo Sapiens 
2023-12-08 | PXD047691 |
TranscriptomicsDifferentiating human embryonic stem cells into corneal epithelial-like cells
Transplantation of ex vivo expanded limbal stem cells (LSC) is the main treatment for limbal stem cell deficiency though the clinical problem of donor tissues shortage. Recently, as the development of tissue engineering, embryonic stem cells (ESC) derived corneal epithelial-like cells (ESC-CEC) has ...
ORGANISM(S): Homo sapiens 
2015-06-24 | E-GEOD-70152 | biostudies-arrayexpress
ProteomicsRapid and in-depth proteomic profiling of small extracellular vesicles for ultralow samples
The form and function of extracellular vesicles (EV) is defined by their proteome. This knowledge is essential to describe and understand EVs, encompassing their marker proteins, capacity as a signalling platform, and utility as diagnostic tools and therapeutic targets. However, EV are low-abundant ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-08-16 | MSV000092668 | MassIVE
Proteomicsintercardiac Phosphoproteome/proteome regulation of cardiomyocyte-fibroblast signaling
Secretome-mediated signaling from human iPSC-derived cardiomyocytes (TGF-beta induced dysfunction) to primary human cardiac fibroblasts was investigated to identify downstream regulators of fibrosis. Quantitative proteomic profiling revealed a dynamic reprogramming of fibroblast global proteome, wit...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-06-27 | MSV000092293 | MassIVE
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