Sort   by:  
 Page size 
ProteomicsIdentification of NEDD8 sites at endogenous levels of NEDD8 expression upon NEDP1 (SENP8, DEN1) knockout.
We developed a strategy that allows the identification of NEDP1 dependent NEDDylation sites under endogenous expression of wild type NEDD8. We combined the use of anti-diGly antibodies that recognise both ubiquitin and NEDD8 modified peptides upon trypsin digestion with short treatment of cells with...
ORGANISM(S): Homo sapiens (Human) 
2023-05-10 | PXD040466 | Pride
Antibody Digly Inhibitor Nedd8 Neddylation Uaei Anti-digly antibodies Ubiquitin 
ProteomicsProteome-wide identification of NEDD8 modification sites reveals distinct proteomes for canonical and atypical NEDDylation
The Ubiquitin-like molecule NEDD8 controls several biological processes and is a promising target for therapeutic intervention. NEDDylation occurs through specific NEDD8 enzymes (canonical) or enzymes of the Ubiquitin system (atypical). Identification of NEDD8 sites on substrates is critical for del...
ORGANISM(S): Homo sapiens (Human) 
2021-02-10 | PXD022812 | Pride
Proteomics Sumo-2 Nucleolus-related inclusions Digly remnants Nedd8 Nedd8 polymers 
ProteomicsSILAC diGlycine proteomics in C. elegans to identify ULP-3 targets upon DNA damage.
We found a conserved role for the deNEDDylating enzyme NEDP1 (ULP-3 in C. elegans) in the DNA damage induced apoptosis. To gain mechanistic insights into the role of ULP-3 in the IR-induced apoptosis, we devised an unbiased proteomics approach to discover potential NEDD8 targets for ULP-3 upon DNA d...
ORGANISM(S): Nematodes (ncbitaxon:333870) 
2019-08-19 | MSV000084214 | MassIVE
ProteomicsMudit analyses of the proteins substrates candidate of NRBP1 using TR-TUBE system
293 stable cell line expressing NRBP1 or NRBP1 mutant were transfected with HA-TR-TUBE using TR-TUBE system to protect the degradation of the ubiquitin chains of the potential ligase substrate. The complexes were pulled down using anti-HA antibody and subsequently digested with modified Trypsin. Ant...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-10-04 | MSV000084419 | MassIVE
ProteomicsUbiquitination in treated T. cruzi parasites
T. cruzi epimastigotes in the logarithmic growth phase (3×106 cells mL-1) were incubated for 12h with bortezomib (1.8 µM), GNF6702 (2.9 µM) or compound 1 (24 µM), equivalent to 8× the EC50 values of each compound. Controls were incubated in the presence of diluent (DMSO). Cells were harvested by cen...
ORGANISM(S): Trypanosoma cruzi Dm28c 
2022-02-17 | PXD027524 | Pride
Ubiquitination Trypanosoma cruzi Proteasome 
Sort   by:  
 Page size