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ProteomicsA commercial antibody to the human condensin II subunit NCAPH2 cross-reacts with a SWI/SNF complex component
Condensin complexes compact and disentangle chromosomes in preparation for cell division. Commercially available antibodies raised against condensin subunits have been widely used to characterise their cellular interactome. Here we have assessed the specificity of a polyclonal antibody (Bethyl A302-...
ORGANISM(S): Mus musculus (Mouse) 
2020-12-14 | PXD022664 | Pride
Swi/snf Condensin Antibody Ncaph2 Cross-react 
ProteomicsLeveraging complementary ion activation methods with proton transfer charge reduction reactions for comprehensive characterization of monoclonal antibody heavy chain subunits v2
Application of gas-phase fractionation and PTCR on the LC-MS timescale to characterize antibody subunits
ORGANISM(S): Homo Sapiens (human) Saccharomyces Cerevisiae (baker's Yeast) 
2025-05-25 | PXD064275 | JPOST Repository
GenomicsOccupancy of Integrator subunits on DNA after hyperosmotic stress
Occupancy of Integrator subunits on DNA after hyperosmotic stress
PRJNA667905 | ENA
ProteomicsMethylated-antibody affinity purification to improve proteomic identification of plant RNA polymerase Pol V complex and the interacting proteins
Affinity purification followed by enzymatic digestion and mass spectrometry has been widely utilized for the sensitive detection of interacting proteins and protein complexes in various organisms. In plants, the method is technically challenging due to the low abundance proteins, non-specific bindin...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2017-02-28 | PXD005422 | Pride
On-bead digestion Pol v complex Chemical cross-link Reductive methylation Ap-ms 
UnknownAlternative splicing for tuneable expression of protein subunits at desired ratios.
Not available
S-EPMC11042056 | biostudies-literature
ProteomicsDe Novo Sequencing of Polyclonal Antibodies by Integrating Intact Mass, Top-Down and Bottom-Up Mass Spectrometry
Antibodies are specialized proteins produced by the adaptive immune system to identify and neutralize harmful antigens. Antibody-based diagnostics and therapeutics have advanced rapidly, with recombinant antibodies derived from monoclonal antibodies (mAbs) becoming the preferred standard due to thei...
ORGANISM(S): Homo sapiens (Human) 
2025-10-31 | PXD063526 | Pride
Intact mass De novo sequencing Top-down Bottom-up Polyclonal antibody sequencing 
GenomicsChIP-seq of Mediator subunits in Mediator mutants and wild type yeast
Mediator subunits Med17 (Srb4) and Med15 (Gal11) were subjected to ChIP in wild type yeast, med18 (srb5), med20 (srb2), and med2 med3 med15 (triple mutant) yeast, all in kin28-anchor away yeast after 1 hr rapamycin treatment to evict Kin28 from the nucleus. This results in inactivation of Kin28, wh...
ORGANISM(S): Saccharomyces cerevisiae 
2017-12-01 | E-MTAB-5824 | biostudies-arrayexpress
UnknownGeneration of an antibody recognizing a set of acetylated proteins, including subunits of BAF complexes.
Not available
S-EPMC7261705 | biostudies-literature
UnknownNovel Antibodies Targeting MUC1-C Showed Anti-Metastasis and Growth-Inhibitory Effects on Human Breast Cancer Cells.
Not available
S-EPMC7247325 | biostudies-literature
ProteomicsEnhanced payload localization in antibody-drug conjugates using a middle-down mass spectrometry approach with proton transfer charge reduction
Here, we present an MD MS strategy relying on the use of PTCR to investigate an ADC with variable drug-to-antibody ratio, targeting the unambiguous localization of payload conjugation sites. Unlike traditional tandem MS experiments (MS2), which were largely ineffective, a single PTCR-based experimen...
ORGANISM(S): Antibody Drug Conjugate 
2024-06-24 | MSV000095128 | MassIVE
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