Sort   by:  
 Page size 
TranscriptomicsNucleosome eviction in mitosis assists condensin loading and chromosome condensation
The experiment was performed to assess the importance of nucleosome eviction for the binding of condensin to chromatin during mitosis. The condensin complex associates with chromatin during mitosis to ensure chromosome condensation and accurate segregation, but how this binding is achieved remains ...
ORGANISM(S): Schizosaccharomyces pombe 972h- 
2016-04-18 | E-MTAB-4620 | biostudies-arrayexpress
GenomicsCondensin association with chromosomes depends on a DNA binding domain formed by its HEAT-repeat subunits
Condensin protein complexes play central roles in the three-dimensional organization of chromosomes during mitotic and meiotic cell divisions. How condensin interacts with its chromatin substrates to promote sister chromatid decatenation and segregation is largely unknown. Previous work suggested th...
ORGANISM(S): Saccharomyces cerevisiae 
2014-05-16 | E-GEOD-55948 | biostudies-arrayexpress
Proteomics3D topology of the SMC2/SMC4 sub-complex from chicken condensin I revealed by cross-linking and molecular modelling
SMC proteins are essential components of three protein complexes that are important for chromosome structure and function. The cohesin complex holds replicated sister chromatids together, whereas the condensin complex has an essential role in mitotic chromosome architecture. Both are involved in int...
ORGANISM(S): Gallus gallus (Chicken) 
2015-07-13 | PXD001835 | Pride
Condensin Cross-linking Mass spectrometry Smc Structure Coiled-coil 
GenomicsGenome-wide analysis of condensin binding sites in vertebrate genome
The condensin complex is well known to be essential for correct packaging and segregation of chromosomes during mitosis and meiosis in all eukaryotes. To date, the genome wide location and the nature of condensin binding sites has remained elusive in vertebrate systems. A detailed knowledge of conde...
ORGANISM(S): Gallus gallus 
2013-10-03 | E-GEOD-45552 | biostudies-arrayexpress
ProteomicsCondensin II Activation by the centromeric protein M18BP1
Condensin I and condensin II promote drastic spatial compaction (condensation) of the genome upon mitotic entry. Condensin I binds chromatin only after dissolution of the nuclear envelope in prophase. Conversely, condensin II is nuclear throughout the cell cycle and initiates chromosome condensation...
ORGANISM(S): Homo sapiens (Human) 
2025-07-09 | PXD051886 | Pride
Sda Sulfo-sda Condensin Cryo-em Xl-ms M18bp1 Crosslinking 
ProteomicsInteraction mapping of human condensin I and condensin II complexes using DSBU-cross-linking and mass spectrometry
Human condensin I and condensin II complexes play essential roles in chromatin condensation during the cell division process. Both complexes use ATP as the energy source to pack extended chromatin into the short form of chromosome. In order to understand the mechanism of their function, we performed...
ORGANISM(S): Homo sapiens (Human) 
2020-05-25 | PXD017531 | Pride
Condensin Dsbu Q-exactive Cross-linking Human Protein complex Xl-ms Chromatin Chromosome 
ProteomicsCryo-EM structures of holo condensin reveal a subunit flip-flop mechanism
This data is from sulfo-SDA crosslinked condensin pentamer. Two datsets, one without atp aand one with ATP. How protein complexes of the SMC family fold DNA into the large loops that are fundamental for the 3D organization of genomes is a central unresolved question of chromosome biology. We used el...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2020-07-28 | PXD019274 | Pride
Condensin Bs3 Crosslinking 
TranscriptomicsCondensin plays a role in both interphase nuclei and premature chromosome condensation
Purpose: to identify how condensin I removal affects gene expression globally. Methods: Chicken DT40 CAP-H KO cells were treated with or without dox for 36 h. Total RNA samples were extracted and subjected to sequencing using an Illumina Hiseq2000 platform. Library preparation and Illumina sequencin...
ORGANISM(S): Gallus gallus 
2017-05-03 | E-GEOD-74892 | biostudies-arrayexpress
ProteomicsCondensin chromatin association is regulated by the combined actions of SMC head and hinge engagement and phosphorylation
The SMC condensin complex is essential for normal mitotic chromosome structure in eukaryotes. Here we undertake a comprehensive analysis of the importance of the condensin ATP binding and hydrolysis, SMC hinge stability and condensin subunit phosphorylation for chromatin association in budding yeast...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2025-10-14 | PXD006028 | Pride
Cerevisiae yeast condensin phosphorylation 
GenomicsIdentification of a novel Bromodomain / Casein Kinase II / TAF-containing complex as a regulator of mitotic condensin function
ABSTRACT: Condensin is a central regulator of mitotic genome structure, with mutants showing poorly condensed chromosomes and profound segregation defects. Here we identify the fission yeast NCT complex, comprising the Nrc1 BET-family tandem bromodomain protein (SPAC631.02), Casein Kinase II (CKII) ...
ORGANISM(S): Schizosaccharomyces pombe 
2014-01-10 | E-GEOD-53955 | biostudies-arrayexpress
Sort   by:  
 Page size