Proteomics

Dataset Information

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Interaction mapping of human condensin I and condensin II complexes using DSBU-cross-linking and mass spectrometry


ABSTRACT: Human condensin I and condensin II complexes play essential roles in chromatin condensation during the cell division process. Both complexes use ATP as the energy source to pack extended chromatin into the short form of chromosome. In order to understand the mechanism of their function, we performed cross-linking mass spectrometry (XL-MS) analyses of both complexes and mapped the inter-molecular and intra-molecular interactions of the subunits.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Dongqing Pan  

LAB HEAD: Andrea Musacchio

PROVIDER: PXD017531 | Pride | 2020-05-25

REPOSITORIES: Pride

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Publications

Human Condensin I and II Drive Extensive ATP-Dependent Compaction of Nucleosome-Bound DNA.

Kong Muwen M   Cutts Erin E EE   Pan Dongqing D   Beuron Fabienne F   Kaliyappan Thangavelu T   Xue Chaoyou C   Morris Edward P EP   Musacchio Andrea A   Vannini Alessandro A   Greene Eric C EC  

Molecular cell 20200522 1


Structural maintenance of chromosomes (SMC) complexes are essential for genome organization from bacteria to humans, but their mechanisms of action remain poorly understood. Here, we characterize human SMC complexes condensin I and II and unveil the architecture of the human condensin II complex, revealing two putative DNA-entrapment sites. Using single-molecule imaging, we demonstrate that both condensin I and II exhibit ATP-dependent motor activity and promote extensive and reversible compacti  ...[more]

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