We carried out 4 HDX-MS experiments to investigate the ARMH3 interactions with PI4Kb, Arl5b(GTPyS) and GBF1 (ARMH3+Arl5b, ARMH3+GBF1(1-709)) and ARMH3+GBF1(364-395)). Analysis of these four experiments defined the Arl5b intereface on ARMH3 and defined the disordered linker region located in the DCB-...
To identify the putative interface between PI4KB and c10orf76, we employed hydrogen–deuterium exchange mass spectrometry (HDX‐MS) to map regions protected in both proteins upon complex formation.
Label free mass spectrometry analysis to investigate the ARL5-dependent function of ARMH3 at the Golgi complex, using MitoID with MTS-BioID2-ARMH3 and MTS-BioID2 (control) constructs.
