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ProteomicsParallel accumulation – serial fragmentation combined with data-independent acquisition (diaPASEF)
Data independent acquisition modes isolate and concurrently fragment populations of different precursors by cycling through segments of a predefined precursor m/z range. Although these selection windows collectively cover the entire m/z range, overall only a few percent of all incoming ions in each ...
ORGANISM(S): Homo sapiens (Human) Saccharomyces cerevisiae (Baker's yeast) 
2020-12-17 | PXD017703 | Pride
Tims Pasef Dia Technical Ion mobility 
ProteomicsOnline parallel accumulation – serial fragmentation (PASEF) with a novel trapped ion mobility mass spectrometer
In bottom-up proteomics, peptides are separated by liquid chromatography with elution peak widths in the range of seconds, while mass spectra are acquired in about 100 microseconds with time-of-fight (TOF) instruments. This allows adding ion mobility as a third dimension of separation. Among several...
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2018-11-06 | PXD010012 | Pride
Tims Pasef Technological 
ProteomicsA novel LC system embeds analytes in pre-formed gradients for rapid, ultra-robust proteomics
To further integrate mass spectrometry (MS)-based proteomics into biomedical research and especially into clinical settings, high throughput and robustness are essential requirements. They are largely met in high-flow rate chromatographic systems for small molecules but these are not sufficiently se...
ORGANISM(S): Homo sapiens (Human) 
2018-08-16 | PXD010393 | Pride
Clinic Plasma Liquid chromatography 
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