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Transcriptional profiling of C. elegans nasp-1 / btr-1 mutant worms versus wild type N2 strain, both exposed to the bacterial pathogen Bacillus thuringiensis DB27. One-condition experiment. C. elegans nasp-1 / btr-1 mutant versus N2, exposed to Bacillus thuringiensis DB27. 3 biological replicates, i...
ORGANISM(S): Caenorhabditis elegans 
Transcriptional profiling of C. elegans young adult worms exposed to pathogen Bacillus thuringiensis DB27 for 4 hours versus age-matched worms exposed to onctrol lab food E. coli OP50. The goal was to identify genes regulated in response to pathogen. The broader goal of study was to study evolution ...
ORGANISM(S): Caenorhabditis elegans 
B. thuringiensis 407 nprA::lacZ strain was compared with an isogenic delta-nprR-nprX strain to explore the effects of nprR-nprX deletion. NprR is a transition state regulator which is activated towards the end of the exponential phase by a part of the signaling peptide NprX. We made direct compariso...
ORGANISM(S): Bacillus thuringiensis Bt407 
We investigated the gene expression and metabolic regulatory mechanisms associated with the high-level accumulation of ICPs by performing the transcriptomics analysis of B. thuringiensis strain CT-43, using Illumina high throughout sequencing (RNA-seq) technique. The bacterial cells were collected a...
ORGANISM(S): Bacillus thuringiensis 
In Bacillus thuringiensis CT-43, five insecticidal crystal proteins (ICPs, Cry protein) are encoded. We extracted the Cry proteins, ran the SDS PAGE (two Cry protein bands were observed), and tried to identify the composition of the two Cry protein bands in the SDS PAGE. The bioinformatics pipeline ...
ORGANISM(S): Bacillus thuringiensis 
2020-01-24 | PXD000425 | Pride
Bacillus thuringiensis strain:SCG04-02 Genome sequencing and assembly
Bacillus thuringiensis israelensis (Bti) toxins are increasingly used for mosquito control, but little is known about the precise mode of action of each of these toxins, and how they interact to kill mosquito larvae. By using RNA sequencing, we investigated change in gene transcription level and pol...
ORGANISM(S): Aedes aegypti 
Comparison in late exponential phase (culture OD600 = 3) of global expression profiles from a Bacillus thuringiensis 407 strain overexpressing transcriptional regulator MogR from xylose inducible vector pHT304-Pxyl, versus an isogenic empty vector control strain, to analyze global expression changes...
ORGANISM(S): Bacillus thuringiensis 
The transcriptional global profiles of the wild-type strain Bacillus thuringiensis 407 and a cdgF deletion mutant were compared after 1.5 hours of growth in an planktonic culture to analyze the effect of deletion of cdgF, which is involved in c-di-GMP signaling, on the global expression profile of B...
ORGANISM(S): Bacillus thuringiensis Bt407 
To determine Sigma 54 (SigL) reglons in Bacillus thuringiensis HD73 strain, A sigLmutant, HD(M-NM-^TsigL::kan), was constructed with insertion of kanamycin resistance gene cassete. We have employed whole genome microarray expression profiling as a discovery platform to identify the difference of gen...
ORGANISM(S): Bacillus thuringiensis 
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