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ProteomicsAffinity capture of S. cerevisiae protein after glutaraldehyde stabilization
It remains extraordinarily challenging to elucidate endogenous protein-protein interactions and proximities within the cellular milieu. The dynamic nature and the large range of affinities of these interactions augment the difficulty of this undertaking. Among the most useful tools for extracting su...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2018-10-19 | PXD001262 | Pride
Budding yeast Glutaraldehyde Affinity capture 
TranscriptomicsMusashi 2 regulates normal hematopoiesis and accelerates leukemogenesis (LK and MS12-inducible)
We demonstrate that Msi2 is the predominant form expressed in hematopoietic stem cells (HSC), and its knockdown leads to reduced engraftment and depletion of HSCs in vivo. Overexpression of Msi2 in a mouse model increases HSC cell cycle progression and cooperates with BCR-ABL1 to induce an aggressiv...
ORGANISM(S): Mus musculus 
2010-07-21 | E-GEOD-22773 | biostudies-arrayexpress
TranscriptomicsMusashi 2 regulates normal hematopoiesis and accelerates leukemogenesis (LSK and LK)
We demonstrate that Msi2 is the predominant form expressed in hematopoietic stem cells (HSC), and its knockdown leads to reduced engraftment and depletion of HSCs in vivo. Overexpression of Msi2 in a mouse model increases HSC cell cycle progression and cooperates with BCR-ABL1 to induce an aggressiv...
ORGANISM(S): Mus musculus 
2010-07-22 | E-GEOD-22774 | biostudies-arrayexpress
TranscriptomicsMusashi 2 regulates normal hematopoiesis and accelerates leukemogenesis (Leukemia cell lines)
We demonstrate that Msi2 is the predominant form expressed in hematopoietic stem cells (HSC), and its knockdown leads to reduced engraftment and depletion of HSCs in vivo. Overexpression of Msi2 in a mouse model increases HSC cell cycle progression and cooperates with BCR-ABL1 to induce an aggressiv...
ORGANISM(S): Homo sapiens 
2010-07-21 | E-GEOD-22775 | biostudies-arrayexpress
TranscriptomicsHuman Mammary Epithelial Cell Response to EGF, microarray analysis
To understand how integration of multiple data types can help decipher cellular responses at the systems level, we analyzed the mitogenic response of human mammary epithelial cells to epidermal growth factor (EGF) using whole genome microarrays, mass spectrometry-based proteomics and large-scale wes...
ORGANISM(S): Homo sapiens 
2010-04-14 | E-GEOD-15668 | biostudies-arrayexpress
TranscriptomicsNext-Generation Sequencing for Whole Transcriptome Analysis in Scrambled Control and EGFR Knockdown Cells Cultured under Normoxia and Hypoxia
Purpose: The goal of this study is to identify the mRNA clusters that are regulated by EGFR under normoxia or hypoxia. Method: Total RNAs were extracted from HeLa cells expressing scrambled control or EGFR shRNA-E1 that cultured under normoxia or hypoxia (1% O2) for 24h. Customized Next-Generation R...
ORGANISM(S): Homo sapiens 
2013-10-01 | E-GEOD-44801 | biostudies-arrayexpress
TranscriptomicsA Proneural to Mesenchymal Transition Mediated by NFkB Promotes Radiation Resistance in Glioblastoma
This SuperSeries is composed of the SubSeries listed below. Refer to individual Series
ORGANISM(S): Homo sapiens 
2013-08-15 | E-GEOD-49009 | biostudies-arrayexpress
ProteomicsMudPIT analyses of the proteins co-purified with wild type-Hzg-3XHA/Flag and phosphatase dead-Hzg-3XHA/Flag affinity purifications from Drosophila melanogaster S2 cells
Protein Complexes Purification- Stable S2 cell expressing Hzg albeit low level, lines expressing C-terminally 3XHA-Flag-tagged wild-type and phosphatase dead (PD) Hzg were generated. A parental cell line was used as a negative control. Nuclear and cytoplasmic extracts from the Hzg-WT, Hzg-mutant and...
ORGANISM(S): Drosophila Melanogaster (ncbitaxon:7227) 
2019-01-08 | MSV000083305 | MassIVE
TranscriptomicsNext-Generation Sequencing for Small RNA Application in Scrambled Control and EGFR Knockdown Cells Cultured under Normoxia and Hypoxia
Purpose: The goal of this study is to identify the miRNA clusters that are regulated by EGFR under normoxia or hypoxia. Method: Total RNAs were extracted from HeLa cells expressing scrambled control or EGFR shRNA-E1 that cultured under normoxia or hypoxia (1% O2) for 24h. Customized Next-Generation ...
ORGANISM(S): Homo sapiens 
2013-10-01 | E-GEOD-44802 | biostudies-arrayexpress
UnknownCalculation of ATP production rates using the Seahorse XF Analyzer
Not available
S-SCDT-10_15252-EMBR_202256380 | biostudies-other
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