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TranscriptomicsInhibition of BET bromodomain proteins as a therapeutic approach in prostate cancer
We analyzed transcriptional changes in 4 prostate cancer cell lines following treatment with the BET inhibitor I-BET762 using Affymetrix Human Genome U133 Plus 2.0 Arrays. Four prostate cancer cell lines (NCI-H660, VCaP, LNCaP, PC-3) were treated with DMSO (control) or I-BET762 at 0.5uM or 10uM con...
ORGANISM(S): Homo sapiens 
2014-03-31 | E-GEOD-56352 | biostudies-arrayexpress
ProteomicsA Chemical Acetylation based Mass Spectrometry Platform for Histone Profiling
Here we present a strategy for the identification and quantitation of histone methylation that utilizes nondeuterated acetic anhydride to fully acetylate all free lysine residues prior to digestion with trypsin and analysis by LC-MS/MS.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
MSV000086146 | MassIVE
TranscriptomicsTreatment of myeloma cell lines with the bromodomain inhibitor I-BET151
KMS12BM and H929 myeloma cells were treated in vitro with either I-BET151 1uM or vehicle (DMSO) for 6 hours (n=3 independent experiments for each cell line). Total RNA was extracted from H929 and KMS12BM cells using the RNeasy Mini kit (Qiagen). Genomic DNA contamination was eliminated using colum...
ORGANISM(S): Homo sapiens 
2013-12-22 | E-MTAB-2122 | biostudies-arrayexpress
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