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MetabolomicsSex-specific effects of CD248 on metabolism and the adipose tissue lipidome

Cd248 has recently been associated with adipose tissue physiology, demonstrated by reduced weight gain in high fat diet-fed mice with genetic deletion of Cd248 relative to controls. Here we set out to determine the metabolic consequences of loss of Cd248. Strikingly, we find these to be sex speci...

2023-01-05 | MTBLS6615 | MetaboLights
TranscriptomicsRNA-seq of human intestinal organoids infected with Salmonella enterica at 2.5h and 8h post infection
The purpose of this experiment was to identify intestinal epithelial responses to various strains of Salmonella enterica. Human intestinal organoids were infected with three serovars of Salmonella; Typhimurium, Enteritidis and Typhi, as well as type 3 secretion system -1 and -2 mutants in Typhimuriu...
ORGANISM(S): Homo sapiens 
2021-10-01 | E-MTAB-10451 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of mouse embryonic stem (ES) cells differentiated for 6 days samplesed at 24 hour timepoints (d1-d6) vs undifferentiated cells (d0)
Mouse ES cells were differentiated for 6 days. Undifferentiated cells (d0) were compared to cells harvested at 24 hour timepoints (d1-d6).
ORGANISM(S): Mus musculus 
2005-08-31 | E-MEXP-304 | biostudies-arrayexpress
TranscriptomicsCell host-response to infection with novel human coronavirus EMC predict potential antivirals and important differences with SARS-coronavirus.
Differential expression was determined in Calu-3 cells between mock infected and infection with either Human coronavirus EMC and SARS coronavirus at different times post infection. Calu-3 2B4 cells were infected with Human Coronavirus EMC 2012 (HCoV-EMC) or mock infected. Samples were collected 0, ...
ORGANISM(S): Homo sapiens 
2013-05-03 | E-GEOD-45042 | biostudies-arrayexpress
ProteomicsRapid and Sensitive Detection of SARS-CoV-2 Infection Using Quantitative Peptide Enrichment Analysis
Reliable, robust, large-scale molecular testing for SARS-CoV-2 is essential for monitoring the ongoing Covid-19 pandemic. We have developed a scalable analytical approach to detect viral proteins based on peptide immunoaffinity enrichment combined with liquid chromatography - mass spectrometry (LC-M...
ORGANISM(S): Homo Sapiens Severe Acute Respiratory Syndrome Coronavirus 2 
2021-11-15 | PXD026366 | panorama
TranscriptomicsEffects of Carbon Nanotubes Substrates on dendritic cells
To assess the effect of carbon nanotubes substrated on dendritic cell (DCs) properties, DCs were generated from human monocytes of healthy donors as previously described (Aldinucci A et al, 2010). In particular, isolated monocytes were cultured for 6 days in medium supplemented with GM-CSF (1000U/ml...
ORGANISM(S): Homo sapiens 
2015-04-01 | E-MEXP-3560 | biostudies-arrayexpress
TranscriptomicsIFN-alpha- and IFN-gamma-regulated transcription in MEFs derived from wild type mice or littermates expressing STAT1F77A
Transcriptional profiling of mouse primary embryonic fibroblasts (MEFs) from wild type (WT) and knockin littermates expressing STAT1F77A (KI). For both genotypes, untreated control cells were compared to cells treated with IFN-alpha or IFN-gamma. Two condition experiments; untreated versus IFN trea...
ORGANISM(S): Mus musculus 
2014-01-12 | E-GEOD-49441 | biostudies-arrayexpress
TranscriptomicsRNA-seq of human intestinal organoids co-cultured with human PMNs infected with Salmonella enterica serovar Typhimurium and Enteritidis at 8h post infection
The purpose of this experiment was to identify the contribution of PMNs in intestinal epithelial responses to Salmonella infection.
ORGANISM(S): Homo sapiens 
2022-12-02 | E-MTAB-11089 | biostudies-arrayexpress
TranscriptomicsTranscription profiling by array of hMADS cells transfected with miR-26a
Human multipotent adipose-derived stem (hMADS) cells were transfected at confluence with miR-26a or miR-C (non-targeting control microRNA) and cells were harvested two days later (=d0) to identify mRNAs that are downregulated by miR-26a versus miR-C, as such mRNAs could be direct miR-26a targets.
ORGANISM(S): Homo sapiens 
2013-11-25 | E-MARS-28 | biostudies-arrayexpress
TranscriptomicsTranscription profiling by array of hMADS cells transfected with miR-26a and induced to differentiate into adipocytes
Human multipotent adipose-derived stem (hMADS) cells were transfected at confluence with miR-26a or miR-C (non-targeting control microRNA). Two days later (=d0), adipocyte differentiation was induced. At d9 after start of differentiation, cells were harvested to identify mRNAs that are down- and upr...
ORGANISM(S): Homo sapiens 
2013-11-25 | E-MARS-29 | biostudies-arrayexpress
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