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ProteomicsExtensive OMICS resources for Sf21 and High Five cell lines
Insect derived cell-lines, from Spodoptera frugiperda (Sf21) and from Trichoplusia ni (High Five), are ones of the most widely used systems for recombinant protein expression in Baculoviral Expression Vector System (BEVS). Genomic sequences and annotations are still incomplete for Sf21 or absent for...
ORGANISM(S): Trichoplusia ni Spodoptera frugiperda 
2022-03-03 | PXD010282 | Pride
High five Transcriptomic Sf21 Insect cell lines Genomic Proteomic 
TranscriptomicsmiRNA profiling from freshly harvested livers
Correlation of miRNA expression patterns with clinically relevant information is greatly facilitated by the retrospective analysis of samples archived in tissue banks. Unfortunately, the quality of samples stored in tissue banks is variable due to heterogeneity in pre-analytical preparation of clini...
ORGANISM(S): Mus musculus 
2010-06-20 | E-GEOD-18775 | biostudies-arrayexpress
TranscriptomicsmiRNA profiling from freshly harvested duodenums
Correlation of miRNA expression patterns with clinically relevant information is greatly facilitated by the retrospective analysis of samples archived in tissue banks. Unfortunately, the quality of samples stored in tissue banks is variable due to heterogeneity in pre-analytical preparation of clini...
ORGANISM(S): Mus musculus 
2010-06-20 | E-GEOD-18774 | biostudies-arrayexpress
GenomicsChIP-exo analysis of the DNA-binding sites of the yeast transcription factor Yfl052w sequenced by Illumina
We determined DNA-binding sites of the yeast transcription factor Yfl052w by ChIP-exo. Cells were grown in the YP media containing palatinose. Yfl052w was tagged with HA tag and anti-HA antibody was used for the immunoprecipitation. Examination of Yfl052 trancription factor in HA-tagged and wt cells...
ORGANISM(S): Saccharomyces cerevisiae 
2014-07-24 | E-GEOD-57900 | biostudies-arrayexpress
TranscriptomicsmiRNA degradation in frozen tissue
To determine the effect of RNA degradation on miRNA expression profiling. To mimic total RNA degradation in snap-frozen livers and duodenums from mice (wild type female, C57/Bl6) tissues were stored in a -80°C freezer and then transferred to dry ice. Each frozen tissue was sliced into five identica...
ORGANISM(S): Mus musculus 
2010-05-19 | E-GEOD-19104 | biostudies-arrayexpress
TranscriptomicsAzacytidine time course on MCF7 cells
MCF7 cells at 80% confluency were treated with 5 micromolar azacytidine with gene expression profiling determined at 0, 1, 2, 3, 4, 6 and 12 hours of exposure to compound. Keywords: time course Approximately 5,000,000 million cells were plated into 10 cm plates from a common pool. Cells were grown i...
ORGANISM(S): Homo sapiens 
2008-01-11 | E-GEOD-10145 | biostudies-arrayexpress
TranscriptomicsRNA-Seq data of Trpm7+/+ and Trpm7-/- embryonic stem cell clones
Partial exon deletion in Trpm7 gene was confirmed by lack of transcripts according to the RNA sequencing in two stem cell clones. For this mice were genetically modified by partial deletion of exon 2 and embryonic stem (ES) cell lines were generated. Isolated RNA of two ES clones was sequenced with ...
ORGANISM(S): Mus musculus 
2021-09-06 | E-MTAB-10877 | biostudies-arrayexpress
TranscriptomicsSaccharomyces cerevisiae 3' poly(A) site mapping
The use of alternative polyadenylation sites is common and affects the post-transcriptional fate of mRNA, including its stability, localization, and translation. Here we present a method for genome-wide and strand-specific mapping of poly(A) sites and quantification of RNA levels at unprecedented ef...
ORGANISM(S): Saccharomyces cerevisiae 
2013-01-10 | E-GEOD-40110 | biostudies-arrayexpress
TranscriptomicsmRNA composition of IRP1 mRNPs in mouse tissues
Affymetrix microarrays were used to determine the mRNA composition of mRNPs obtained by immunoprecipitation with IRP1 (iron regulatory protein 1). Reference Sanchez at al., 2007 Nat. Protoc. Affymetrix microarrays were used to determine the mRNA composition of mRNPs obtained by immunoprecipitation ...
ORGANISM(S): Mus musculus 
2011-12-09 | E-GEOD-17096 | biostudies-arrayexpress
TranscriptomicsmRNA composition of IRP2 mRNPs in mouse tissues
Affymetrix microarrays were used to determine the mRNA composition of mRNPs obtained by immunoprecipitation with IRP2 (iron regulatory protein 2). Reference Sanchez at al., 2007 Nat. Protoc. Affymetrix microarrays were used to determine the mRNA composition of mRNPs obtained by immunoprecipitation ...
ORGANISM(S): Mus musculus 
2011-12-09 | E-GEOD-17097 | biostudies-arrayexpress
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