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ProteomicsFASIL-MS: An Integrated Proteomic and Bioinformatic Workflow to Universally Quantitate In vivo-acetylated Positional Isomers
Dynamic changes in histone post-translational modifications (PTMs) regulate gene transcription leading to fine-tuning of biological processes such as DNA replication and cell cycle progression. Moreover, specific histone modifications constitute docking sites for recruitment of DNA damage repair pr...
ORGANISM(S): Bos taurus (Bovine) Homo sapiens (Human) 
2016-06-16 | PXD003611 | Pride
Ms2-based quantitation Lysine deacetylase inhibitor Histone acetylation Mass spectrometry N-acetoxy-d3-sucinnimide Fasp Protein derivatisation 
ProteomicsIdentifying Kinase Substrates via a Heavy ATP Kinase Assay and Quantitative Mass Spectrometry
Mass spectrometry‐based in vitro kinase screens play an essential role in the discovery of kinase substrates, however, many suffer from biological and technical noise or necessitate genetically‐altered enzymecofactor systems. We describe a method that combines stable γ‐[18O2]‐ATP with classical in v...
ORGANISM(S): Homo sapiens (Human) 
2018-10-17 | PXD002133 | Pride
Human Kinase substrates  in vitro kinase assay  proteomics  mass spectrometry  heavy atp  non receptor tyrosine kinase abl1 
ProteomicsA surface biotinylation strategy for reproducible plasma membrane protein purification and tracking of genetic and drug-induced alterations
In the past years several protocols for the proteomic profiling of plasma membrane proteins have been described. Nevertheless, comparative analyses have mainly focused on different variations of one approach [1-3]. To pave the way to high-performance differential plasma membrane proteomics, we compa...
ORGANISM(S): Homo sapiens (Human) 
2022-02-22 | PXD002141 | Pride
Kbm7 Mcp Technical paper Cell surface proteomics 
ProteomicsA common cell state in Triple Negative Breast Cancers can be targeted through inhibition of Spleen Tyrosine Kinase (SYK)
Breast tumors are highly heterogeneous and for many molecular subtypes no targeted therapies are available. These include breast cancers that display hallmarks of epithelial to mesenchymal transition (EMT), a process related to metastasis and enriched in triple negative breast cancers (TNBCs). To de...
ORGANISM(S): Homo sapiens (Human) 
2016-06-15 | PXD001680 | Pride
Syk inhibition Phosphotyrosine proteomics Human breast cancer 
ProteomicsMissing proteins identified from the haploid cell lines: HAP1 and KBM-7
Chromosome-centric Human Proteomic Project (C-HPP) aims at identifying and characterizing protein products encoded from all protein-coding genes of human chromosomes. As evident in the recent nature papers, it is possible that those missing proteins may be low abundant in many cell types or expresse...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-06-10 | MSV000083952 | MassIVE
ProteomicsABRF PRG 2012: Longitudinal QC in Routine Peptide LC-MS/MS Analyses
Questions concerning data quality and reproducibility of proteomic laboratories spurred the ABRF-PRG group to design a study to systematically assess the reproducibility of proteomic laboratories. Sixty four participants were recruited from the broader mass spectrometry community and were asked to ...
ORGANISM(S): Bos Taurus (ncbitaxon:9913) 
2015-04-28 | MSV000079117 | MassIVE
TranscriptomicsHDAC1/2 and CD4+ T cells
Dynamic changes in histone acetylation patterns are mediated by the activity of histone acetyltransfereases (HATs) and histone deacetylases (HDACs) and are key events in the epigenetic regulation of gene expression. The application of HDAC inhibitors revealed a variety of T cell functions controlled...
ORGANISM(S): Mus musculus 
2014-03-30 | E-MTAB-2184 | biostudies-arrayexpress
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