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ProteomicsRNA Polymerase II interactome in presence or absence of SCAF1 in U2OS-SCAF1-AID cells
Transcripts produced by RNA polymerase II (RNAPII) are fundamental for cellular responses to environmental changes. It is therefore no surprise that there exist multiple avenues for the regulation of this process. To explore the regulation mediated by RNAPII-interacting proteins, we used an siRNA-ba...
ORGANISM(S): Homo sapiens (Human) 
2024-12-20 | PXD054675 | Pride
Scaf1-depletion Interactome Rnapii 
ProteomicsInteractome of chromatin-associated RNA Polymerase II from U2OS cells
To determine the interactome of RNA Polymerase II (RNAPII), HA-tagged RPB3 was stably expressed in U2OS cells. The cells were harvested followed by the native stepwise isolation of chromatin-associated RNAPII complexes. HA-RPB3 was immunoprecipitated and interacting proteins analyzed by quantitative...
ORGANISM(S): Homo sapiens (Human) 
2024-12-20 | PXD054701 | Pride
Interactome U2os Rnapii 
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