Sort   by:  
 Page size 
ProteomicsHippocampal proteomic profiling following peripheral delivery of klotho fragment
We found that a klotho protein fragment (KL-F), administered peripherally, induced cognitive enhancement and neural resilience. Here we report a proteomic analysis of whole hippocampal homogenates from mice treated with vehicle or KL-F (10 µg/kg, i.p.) four hours prior to exploration in the small Y-...
ORGANISM(S): Mus musculus (Mouse) 
2017-08-09 | PXD007150 | Pride
Hippocampus Dia Swath Biognosys Klotho 
ProteomicsCombining Precursor and Fragment Information for Improved Detection of Differential Abundance in Data Independent Acquisition
In data independent acquisition, consistent, comprehensive MS1 and MS2 can be recorded. This enables statistical comparison based on the two quantitative levels.
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) Homo sapiens (Human) Mus musculus (Mouse) Escherichia coli Saccharomyces cerevisiae (Baker's yeast) Caenorhabditis elegans 
2020-01-07 | PXD016647 | Pride
Controlled data sets Ms1 ms2 quantification Dia 
ProteomicsAnalysis of 1,508 plasma samples of the DiOGenes study - Robust, single shot capillary flow data-independent acquisition to decipher proteomic profiles of weight loss and maintenance
Based on our own experience and the evaluation of recent large-scale plasma mass spectrometry (MS) based proteomic studies (1, 2), we identified two outstanding challenges: slow and delicate nano-flow liquid chromatography (LC) and irreproducibility of identification of data-dependent acquisition (D...
ORGANISM(S): Homo sapiens (Human) 
2019-04-05 | PXD013231 | Pride
Plasma proteomics Swath Library Single shot Clinical proteomics Stable isotope-based quantification High throughput Label-free quantification Data-independent acquisition 
ProteomicsSurpassing 10,000 identified and quantified proteins in a single run by optimizing current LC-MS instrumentation and data analysis strategy
Comprehensive proteome quantification is crucial for a better understanding of underlying mechanisms of diseases. Liquid chromatography mass spectrometry (LC-MS) has become the method of choice for comprehensive proteome quantification due to its power and versatility. Even though great advances hav...
ORGANISM(S): Homo sapiens (Human) 
2019-08-12 | PXD013658 | Pride
Liquid chromatography Dia Testis cancer Hybrid library 
ProteomicsA flexible end-to-end automated sample preparation workflow enables reproducible large-scale bottom-up proteomics - Part 1
Bottom-up proteomics holds significant promise for clinical applications due to its high sensi-tivity and precision, but is limited by labor-intensive, low-throughput sample preparation meth-ods. Advanced automation is essential to enhance throughput, reproducibility, and accuracy and to allow stand...
ORGANISM(S): Homo sapiens (Human) 
2025-10-17 | PXD063610 | Pride
Automated sample preparation End-to-end 
ProteomicsComparison of DIA and TMT based protein quantification in complex background
Label free quantification (LFQ) and isobaric labelling quantification (ILQ) are among the most popular protein quantification workflows in discovery proteomics. Here, we compared the TMT 10-plex workflow to label free single shot data-independent acquisition (DIA) method on a controlled sample set. ...
ORGANISM(S): Mus musculus (Mouse) 
2019-01-24 | PXD011691 | Pride
Tmt Orbitrap fusion lumos Dia 
ProteomicsA flexible end-to-end automated sample preparation workflow enables reproducible large-scale bottom-up proteomics - Part 4
Bottom-up proteomics holds significant promise for clinical applications due to its high sensitivity and precision, but is limited by labor-intensive, low-throughput sample preparation meth-ods. Advanced automation is essential to enhance throughput, reproducibility, and accuracy and to allow standa...
ORGANISM(S): Homo sapiens (Human) 
2025-10-17 | PXD063831 | Pride
Automated sample preparation End-to-end 
ProteomicsA flexible end-to-end automated sample preparation workflow enables reproducible large-scale bottom-up proteomics - Part 2
Bottom-up proteomics holds significant promise for clinical applications due to its high sensitivity and precision, but is limited by labor-intensive, low-throughput sample preparation meth-ods. Advanced automation is essential to enhance throughput, reproducibility, and accuracy and to allow standa...
ORGANISM(S): Homo sapiens (Human) 
2025-10-17 | PXD063636 | Pride
Automated sample preparation End-to-end 
ProteomicsA flexible end-to-end automated sample preparation workflow enables reproducible large-scale bottom-up proteomics - Part 3
Bottom-up proteomics holds significant promise for clinical applications due to its high sensitivity and precision, but is limited by labor-intensive, low-throughput sample preparation meth-ods. Advanced automation is essential to enhance throughput, reproducibility, and accuracy and to allow standa...
ORGANISM(S): Homo sapiens (Human) 
2025-10-17 | PXD063679 | Pride
Automated sample preparation End-to-end 
ProteomicsScreening of candidate proteins affecting spermatogenesis by DIA analysis of testis proteomics in Shandong black cattle
All samples were processed by DIA individually to assess the proteome differences. MS1 and MS2 data were all acquired, and samples acquisition by random order. The iRT kit (Ki3002, Biognosys AG, Switzerland) was added to all of the samples to calibrate the retention time of extracted peptide peaks. ...
ORGANISM(S): Bos taurus (Bovine) 
2025-06-11 | PXD061007 | Pride
Shandong black cattle， testis，dia 
Sort   by:  
 Page size