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Protein synthesis underpins many biological processes, yet tracking time-dependent proteomic changes remains challenging. Bioorthogonal noncanonical amino acid tagging (BONCAT) offers a targeted approach for labeling and identifying newly synthesized proteins within defined time windows of interest....
ORGANISM(S): Danio rerio (Zebrafish) (Brachydanio rerio) 
2026-06-08 | PXD063084 | Pride
Incubation of marine methane seep sediments with a bioorthogonal amino acid
Pulsing the non-canonical amino acid azidohomoalanine into Arabidopsis thaliana seedlings enables in-gel visualization, physical enrichment, and identification of newly synthesized proteins.
ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress) 
Cell-selective bioorthogonal noncanonical amino acid tagging (BONCAT) of newly synthesized proteins, catalyzed by engineered aminoacyl-tRNA synthetase (aaRSs), is a powerful technology for time-resolved characterization of the nascent proteome. Currently, cell-selective BONCAT studies in bacteria re...
ORGANISM(S): Klebsiella pneumoniae ATCC 43816 Escherichia coli Acinetobacter baumannii (strain ATCC 17978 / CIP 53.77 / LMG 1025 / NCDC KC755 / 5377) 
2026-04-11 | PXD067013 | Pride
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