Sort   by:  
 Page size 
ProteomicsRefinement of Protein Extraction Protocols for Human Peripheral Nerve Tissue
Our aim was to establish an effective method for protein extraction from freshly frozen human peripheral nerves and determine the minimum amount required for consistent protein extraction outcomes. Five extraction methods were compared using 8 M Urea and Ripa buffer, using either the Bullet Blender ...
ORGANISM(S): Homo sapiens (Human) 
2025-02-21 | PXD049366 | Pride
Sural nerve Human Extracellular proteins Nerve regeneration Proteomics Lc-ms/ms 
ProteomicsASSESSING AUTOMATED SAMPLE PREPARATION TECHNOLOGIES FOR HIGH-THROUGHPUT PROTEOMICS OF FROZEN WELL CHARACTERIZED TISSUES FROM SWEDISH BIOBANKS
Large cohorts of carefully collected and stored clinical tissue materials play a central role in acquiring sufficient depth and statistical power to discover disease-related mechanisms and biomarkers of clinical significance. Manual preparation of such heterogeneous samples is labor intensive and re...
ORGANISM(S): Homo sapiens (Human) Rattus norvegicus (Rat) 
2018-11-27 | PXD010296 | Pride
Bravo assaymap Tissue Mass spectrometry Clinical proteomics Barocycler Bioruptor 
UnknownRefinement of Protein Extraction Protocols for Human Peripheral Nerve Tissue.
Not available
S-EPMC11822717 | biostudies-literature
GenomicsMapping of ETV1 genomic binding sites in gastrointestinal stromal tumor (GIST).
ETV1 is highly expressed in GIST and their presumed precursors--the interstitial cells of Cajal. ETV1 is required for survival for both GIST and ICC and regulates GIST signature genes. Here, using Illumina-Solexa based next-generation sequencing of ETV1 chromatin immunoprecipates (ChIP-Seq), we defi...
ORGANISM(S): Homo sapiens 
2010-10-14 | E-GEOD-22441 | biostudies-arrayexpress
GenomicsMolecular profiling of Ctcf hemizygous mouse embryonic fibroblasts: ChIP-seq
CTCF is a highly conserved and ubiquitously expressed protein involved in several fundamental processes such as fine-tuning gene expression, imprinting, X-chromosome inactivation and 3D chromatin organisation. To understand the impact of differences in the concentration of CTCF abundance on these pr...
ORGANISM(S): Mus musculus 
2018-07-29 | E-MTAB-6261 | biostudies-arrayexpress
GenomicsLoss of Asxl2 leads to myeloid malignancies in mice. [ChIP-Seq]
LK cells of each mouse genotype were fixed with 1% formaldehyde for 15 min and quenched with 0.125 M glycine. Chromatin was isolated by the addition of lysis buffer, followed by shearing with Bioruptor Pico with water cooler (Diagenode, Seraing, Belgium). The DNA was sheared to an average length of ...
ORGANISM(S): Mus musculus 
2018-03-08 | GSE97020 | GEO
GenomicsGenome-wide replication timing in the mutants deleted for telomere-binding proteins in S. pombe
We report the regions of newly replicated DNA in the presnece of HU in mutants deleted for telomere-binding proteins. Cells were grown at permissive temperature (25°C) in EMM medium for more than 14 hours to induce the expression of TK (thymidine kinase) and then shifeted to 35.5°C for 4 hours to ar...
ORGANISM(S): Schizosaccharomyces pombe 
2019-03-31 | GSE94812 | GEO
ProteomicsASSESSING AUTOMATED SAMPLE PREPARATION TECHNOLOGIES FOR HIGH-THROUGHPUT PROTEOMICS OF FROZEN WELL CHARACTERIZED TISSUES FROM SWEDISH BIOBANKS
Large cohorts of carefully collected and stored clinical tissue materials play a central role in acquiring sufficient depth and statistical power to discover disease-related mechanisms and biomarkers of clinical significance. Manual preparation of such heterogeneous samples is labor intensive and re...
ORGANISM(S): Rattus norvegicus (Rat) 
2018-11-27 | PXD011295 | Pride
Mass spectrometry clinical peoteomics 
TranscriptomicsGenome-wide mapping of the HY5-mediated gene networks in Arabidopsis that involve both transcriptional and post-transcriptional regulations: ChIP-Chip
LONG HYPOCOTYL 5 (HY5) is a basic leucine zipper transcription factor (TF) that functions downstream of multiple families of photoreceptors. Mutations in the HY5 gene cause a myriad of aberrant phenotypes in Arabidopsis, including elongated hypocotyl, reduced accumulation of pigments, halted chlorop...
ORGANISM(S): Arabidopsis thaliana 
2010-12-31 | E-GEOD-24973 | biostudies-arrayexpress
ProteomicsDifferential protein expression between Carfilzomib-sensitive (AMO1) and resistant (AMO1-CFZ) multiple myeloma cells
Carfilzomib-sensitive (AMO1) and resistant (AMO1-CFZ) MM cells were grown in RPMI-1640 medium supplemented with 10 % FBS and 0.68 mM L-glutamine, in a humidified incubator at 5% CO2 and 37 °C. The AMO1-CFZ medium was additionally added 90 nM CFZ, while the CFZ sensitive AMO1 cells were added vehicle...
ORGANISM(S): Homo sapiens (Human) 
2024-06-07 | PXD045439 | Pride
Myeloma 
Sort   by:  
 Page size