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ProteomicsBiomarkers for Cervical Cancer. iTRAQ Discovery.
We developed a discovery-validation mass-spectrometry based pipeline to identify a set of proteins that are regulated in serum of patients with cervical intraepithelial neoplasia (CIN) and squamous cell cervical cancer using isobaric Tags for Relative and Absolute Quantitation (iTRAQ®), label-free ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-07-19 | MSV000079925 | MassIVE
ProteomicsSMD-IPTL 4-plex yeast and BSA-yeast
Isobaric peptide termini labeling (IPTL) is an attractive protein quantification method, because it provides more accurate and reliable quantification information than traditional isobaric labelling methods (TMT, iTRAQ) by making use of the entire fragment ion series instead of only a single reporte...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2020-04-27 | PXD018589 | Pride
Smd-iptl 
ProteomicsAc-IP tag enables multiplexed proteome quantification in data-independent acquisition mode
We propose the isotopically labeled Ac-IP (acetyl-isoleucine-proline) tag for multiplexed quantification of peptides at the MS1 level in the data-independent acquisition (DIA) mode. Differentially labeled peptides have distinct precursor ions carrying the quantitative information but identical MS2 s...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2021-06-07 | PXD022606 | Pride
Quantitative proteomics Isotope labeling Data-independent acquisition 
ProteomicsFixated rat liver tissue - Cleavable crosslinkers as tissue fixation reagents for proteomic analysis
Formaldehyde fixation is widely used for long-term maintenance of tissue. However, due to formaldehyde-induced cross-links, fixated tissue proteins are difficult to extract hampering the performance of mass spectrometry (MS)-proteomic analysis on formaldehyde-fixated tissue. Recent years saw the use...
ORGANISM(S): Rattus norvegicus (Rat) 
2018-01-29 | PXD002169 | Pride
Rat liver Tissue fixation Cleavable crosslinkers Reversible crosslinking 
ProteomicsA Collision-induced Dissociation Cleavable Isobaric Tag for Peptide Fragment Ion-based Quantification in Proteomics
Fragment ion-based proteome quantification methods quantify peptides based on unique peptide fragment ions avoiding the issue of ratio distortion that is commonly observed for reporter ion-based quantification approaches. Herein, we present a novel approach that relies on a collision-induced dissoci...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2020-08-17 | PXD018790 | Pride
Isotopic labelling Proteomics Cid cleavable tag Fragment ion-based quantification Isobaric labelling 
ProteomicsA Versatile Isobaric Tag Enables Proteome Quantification in Data Dependent and Data Independent Acquisition Mode
Quantifying proteins based on peptide-coupled reporter-ions is a leading multiplexed quantitative strategy in proteomics that significantly alleviates the problem of ratio distortion caused by peptide co-fragmentation, as commonly observed in other reporter-ion based approaches, such as TMT and iTRA...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2021-09-09 | PXD021187 | Pride
Quantitative proteomics Data dependent acquisition Isobaric labeling Data-independent acquisition 
ProteomicsBiomarkers for Cervical Cancer. iTRAQ Discovery.
We developed a discovery-validation mass-spectrometry based pipeline to identify a set of proteins that are regulated in serum of patients with cervical intraepithelial neoplasia (CIN) and squamous cell cervical cancer using isobaric Tags for Relative and Absolute Quantitation (iTRAQ®), label-free s...
ORGANISM(S): Homo sapiens (Human) 
2014-10-06 | PXD001034 | Pride
Alpha-1-acid-glycoprotein Protein panel Alpha-1-antitrypsin Vitamin d-binding protein Intraepithelial neoplasia Haptoglobin Serotransferrin Alpha-2-hs-glycoprotein Cervical cancer Lc-ms/ms 
ProteomicsBiomarkers for cervical cancer. Verfification.
We developed a discovery-validation mass-spectrometry based pipeline to identify a set of proteins that are regulated in serum of patients with cervical intraepithelial neoplasia (CIN) and squamous cell cervical cancer using isobaric Tags for Relative and Absolute Quantitation (iTRAQ®), label-free s...
ORGANISM(S): Homo sapiens (Human) 
2014-10-06 | PXD001055 | Pride
Alpha-1-acid-glycoprotein Protein panel Alpha-1-antitrypsin Vitamin d-binding protein Intraepithelial neoplasia Haptoglobin Serotransferrin Alpha-2-hs-glycoprotein Cervical cancer Lc-ms/ms 
ProteomicsUnspecific Binding of Beads to Plasma Proteins
Nickel and maleimide beads were incubated in plasma and analysed with shotgun proteomics to determine the amount of plasma proteins bound to them
ORGANISM(S): Homo sapiens (Human) 
2022-02-17 | PXD027103 | Pride
Unspecif binding plsam proteins 
ProteomicsAssessment of sample preparation bias in mass spectrometry-based proteomics
For mass spectrometry-based proteomics, the selected sample preparation strategy is a key determinant for information that will be obtained. However, the corresponding selection is often not based on a fit-for-purpose evaluation. Here we report a comparison of in-gel (IGD), in-solution (ISD), on-fil...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-03-13 | MSV000083561 | MassIVE
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