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TranscriptomicsSeparation of DNA replication from the assembly of break-competent meiotic chromosomes
The meiotic cell division reduces the chromosome number from diploid to haploid to form gametes for sexual reproduction. Although much progress has been made in understanding meiotic recombination and the two meiotic divisions, the processes leading up to recombination, including the prolonged pre-m...
ORGANISM(S): Saccharomyces cerevisiae 
2012-06-14 | E-GEOD-35667 | biostudies-arrayexpress
TranscriptomicsS phase and HU profiles in wild-type and mutant cells
Total S phase was measured for wild-type cells undergoing meiS and mitS. Early replication origins were mapped in mitS in wild-type cells, and in meiS for wild-type, sml1 delete, rec8 delete and spo11 delete cells. Comparative genome hybridization was used to measure the kinetics and final extent of...
ORGANISM(S): Saccharomyces cerevisiae 
2012-06-18 | E-GEOD-35662 | biostudies-arrayexpress
TranscriptomicsDSB formation in replication compromised cells
DSBs were mapped genome-wide by ssDNA enrichment in cdc6-mn replication comprimsied strains. We mapped DSB sites by detecting DSB-associated ssDNA enrichment on microarrays. To test the role of DNA replication in DSB formation, we mapped ssDNA in a cdc6-mn replication depleted strain. ssDNA was isol...
ORGANISM(S): Saccharomyces cerevisiae 
2012-06-18 | E-GEOD-35666 | biostudies-arrayexpress
TranscriptomicsChromatin IP for Mcm2-7, Rec8, Hop1 and Red1
Mcm2-7 ChIP in pre-meiotic and pre-mitotic cells, axis factor ChIP in wild-type and replication compromised strains in meiosis Multiple studies of meiotic chromosomes were undertaken. To study DNA replication, the locations of replicative helicase (Mcm2-7) were mapped in pre-meiotic and pre-mitotic ...
ORGANISM(S): Saccharomyces cerevisiae 
2012-06-18 | E-GEOD-35658 | biostudies-arrayexpress
TranscriptomicsssDNA mapping in dmc1 strains
ssDNA enrichment was used to map and compare DSB hotspots in dmc1, pch2 dmc1, sir2 dmc1, orc1-161 dmc1, dmc1 rdnadelete and dmc1 chr2:12 translocation strains. 6 samples, 2 replicates each. Averaged data is available as a supplementary file on the Series record (below).
ORGANISM(S): Saccharomyces cerevisiae 
2011-08-14 | E-GEOD-30071 | biostudies-arrayexpress
TranscriptomicsMapping of meiotic double-strand break (DSB) sites and DSB factor association near repetitive DNA elements in S. cerevisiae
DNA double strand breaks (DSBs) in repetitive sequences are a potent source of genomic instability, due to the possibility of non-allelic homologous recombination (NAHR). Repetitive sequences are especially at risk during meiosis, when numerous programmed DSBs are introduced into the genome to initi...
ORGANISM(S): Saccharomyces cerevisiae 
2011-08-14 | E-GEOD-30073 | biostudies-arrayexpress
TranscriptomicsChIP-chip of DSB factors in wild type and pch2 strains
ChIP-chip was used to compare DSB factor localization in wild type and pch2 strains 10 samples, 2-3 replicates each. Averaged data is available as a supplementary file on the Series record (below).
ORGANISM(S): Saccharomyces cerevisiae 
2011-08-14 | E-GEOD-30072 | biostudies-arrayexpress
GenomicsWhole-genome nucleosome mapping in meiotic diploid S. cerevisiae
We determined nucleosome positions throughout the genome in diploid S. cerevisiae undergoing early stages of synchronous meiosis. This study sought to assess if systematic reorganization of nucleosomes occurs during meiotic prophase at or near sites of DNA double strand break formation. Six samples ...
ORGANISM(S): Saccharomyces cerevisiae 
2011-03-07 | E-GEOD-26452 | biostudies-arrayexpress
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