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TranscriptomicsComparative genomic hybridization of kanmycin selected Vibrio cholerae with serogroup 01 and those converted from serogroup 01 to 037
These experiments were performed to show a serogroup conversion in Vibrio cholerae from O1 to O139. For this purpose, V. cholerae O1 WT = A1552 was grown on crab shell fragments to induce natural competence for transformation. Purified DNA (2 ug each) from strain VC73-orf6/7-Kan-A was added after 24...
ORGANISM(S): Vibrio cholerae 
2007-07-25 | E-SMDB-3849 | biostudies-arrayexpress
TranscriptomicsComparative genomic hybridization of Vibrio cholerae serogroup conversion from 01 to 0139
These experiment were performed to show a serogroup conversion in Vibrio cholerae from O1 to O139. For this purpose, V. cholerae O1 WT = A1552 was grown on crab shell fragments to induce natural competence for transformation. Purified DNA (2 ug each) from strain VCO139-Kan was added after 24h and th...
ORGANISM(S): Vibrio cholerae 
2007-08-01 | E-SMDB-3574 | biostudies-arrayexpress
TranscriptomicsComparative genomic hybridization of Vibrio cholerae which have converted from serogroup 01 to 0139 (phage selected)
These experiments were performed to show a serogroup conversion of Vibrio cholerae from O1 to O139. For this purpose, V. cholerae O1 El Tor (A1552) was grown on crab shell fragments to induce natural competence for transformation. Purified DNA (4 ug each) from strain MO10, an O139 serogroup strain, ...
ORGANISM(S): Vibrio cholerae 
2007-08-01 | E-SMDB-3752 | biostudies-arrayexpress
TranscriptomicsComparative genomic hybridization of Vibrio cholerae which have converted from serogroup 01 to 0139 (phage selected) - part 2
These experiments were performed to show a serogroup conversion of Vibrio cholerae from O1 to O139. For this purpose, V. cholerae O1 El Tor (A1552) was grown on crab shell fragments to induce natural competence for transformation. Purified DNA (4 ug each) from strain MO10, an O139 serogroup strain, ...
ORGANISM(S): Vibrio cholerae 
2007-07-25 | E-SMDB-3821 | biostudies-arrayexpress
TranscriptomicsComparative genomic hybridization of Vibrio cholerae serogroup conversion from 01 to 0139 in mixed communities/biofilms
These experiments were performed to show serogroup conversion in Vibrio cholerae from O1 to O139 in a mixed communities / biofilms. For this purpose, V. cholerae O1 El Tor A1552 and VCO139-Kan strain (a MO10 derivative; O139 serogroup) were grown on crab shell fragments to induce natural competence ...
ORGANISM(S): Vibrio cholerae 
2007-07-25 | E-SMDB-3820 | biostudies-arrayexpress
TranscriptomicsDNA microarray analysis of genes positively regulated by TfoX
Vibrio cholerae strains A1552tfoX/pBAD and A1552tfoX/pBAD-tfoX were diluted 1:100 from over night cultures and grown in LB liquid medium containing 0.2 % L-arabinose and 100 μg/ml ampicillin. At OD600=0.3, samples were quickly harvested in a microcentrifuge at room temperature and the bacterial pel...
ORGANISM(S): Vibrio cholerae 
2010-06-30 | E-GEOD-3577 | biostudies-arrayexpress
TranscriptomicsDNA microarray analysis of genes positively regulated by HapR
Vibrio cholerae strains A1552 and ATN140 (A1552-delta-hapR) were diluted 1:100 from over night cultures and grown aerobically (at 250 rpm) in M9 minimal medium supplemented with vitamins (MEM vitamin solution, BRL) and 0.5% lactate. At OD600 ~ 0.3 2 mM (GlcNAc)6 (Seikagaku America, East Falmouth, MA...
ORGANISM(S): Vibrio cholerae 
2010-06-30 | E-GEOD-3576 | biostudies-arrayexpress
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