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Genomic DNA from cell lines was extracted and treated with sodium bisulfite. Fragments were PCR-amplified from the treated template DNA, labelled and hybridized to the microarray. The array contained probe sequences complementary to formerly methylated (M) and unmethylated (U) cytosines respectively...
ORGANISM(S): Homo sapiens 
RNAi screens via pooled short hairpin RNAs (shRNAs) have recently become a powerful tool for the identification of essential genes in mammalian cells. We synthesized DNA microarrays with six overlapping 25 nt long tiling probes complementary to each unique 60 nt molecular barcode sequence associated...
ORGANISM(S): Homo sapiens 
RNAi screens via pooled short hairpin RNAs (shRNAs) have recently become a powerful tool for the identification of essential genes in mammalian cells. We synthesized DNA microarrays with six overlapping 25 nt long tiling probes complementary to each unique 60 nt molecular barcode sequence associated...
ORGANISM(S): Homo sapiens 
RNAi screens via pooled short hairpin RNAs (shRNAs) have recently become a powerful tool for the identification of essential genes in mammalian cells. We synthesized DNA microarrays with six overlapping 25 nt long tiling probes complementary to each unique 60 nt molecular barcode sequence associated...
ORGANISM(S): Homo sapiens 
RNAi screens via pooled short hairpin RNAs (shRNAs) have recently become a powerful tool for the identification of essential genes in mammalian cells. We synthesized DNA microarrays with six overlapping 25 nt long tiling probes complementary to each unique 60 nt molecular barcode sequence associated...
ORGANISM(S): Homo sapiens 
To examine off-target effects of RAD17 RNAi by shRNA transduction, expression profilling was performed.
ORGANISM(S): Homo sapiens 
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