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ProteomicsOnline-2D nanoLC-MS for crude serum proteome profiling: assessing sample preparation impact on proteome composition
Although current LC-MS technology permits scientists to efficiently screen clinical samples in translational research, e.g. steroids, biogenic amines and even plasma or serum proteomes, in a daily routine, maintaining the balance between throughput and analytical depth is still a limiting factor. A ...
ORGANISM(S): Homo sapiens (Human) 
2021-07-09 | PXD024893 | Pride
Peptide fractionation Blood Nanolc-ms 1d nanolc-ms Proteome profiling Crude serum High-resolution accurate mass-spectrometry Online-2d nanolc-ms 
ProteomicsDeep single-shot nanoLCMS proteome profiling with 1500 bar UHPLC system, long fully porous columns, and HRAM MS
This study demonstrates how the latest UHPLC (ultra-high-performance liquid chromatography) technology can be combined with high-resolution accurate-mass (HRAM) mass spectrometry (MS) and long columns packed with fully porous particles to improve bottom-up proteomics analysis with nano-flow liquid c...
ORGANISM(S): Homo sapiens (Human) 
2022-09-12 | PXD035665 | Pride
Human Lcms Cells Orbitrap 
ProteomicsOnline-2D nanoLC-MS for crude serum proteome profiling: assessing sample preparation impact on proteome composition
Although current LC-MS technology permits scientists to efficiently screen clinical samples in translational research, e.g. steroids, biogenic amines and even plasma or serum proteomes, in a daily routine, maintaining the balance between throughput and analytical depth is still a limiting factor. A ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2025-06-27 | MSV000098369 | MassIVE
UnknownRobust Microflow LC-MS/MS for Proteome Analysis: 38 000 Runs and Counting.
Not available
S-EPMC7616893 | biostudies-literature
ProteomicsBiomarkers for Cervical Cancer. iTRAQ Discovery.
We developed a discovery-validation mass-spectrometry based pipeline to identify a set of proteins that are regulated in serum of patients with cervical intraepithelial neoplasia (CIN) and squamous cell cervical cancer using isobaric Tags for Relative and Absolute Quantitation (iTRAQ®), label-free s...
ORGANISM(S): Homo sapiens (Human) 
2014-10-06 | PXD001034 | Pride
Alpha-1-acid-glycoprotein Protein panel Alpha-1-antitrypsin Vitamin d-binding protein Intraepithelial neoplasia Haptoglobin Serotransferrin Alpha-2-hs-glycoprotein Cervical cancer Lc-ms/ms 
ProteomicsBiomarkers for cervical cancer. Verfification.
We developed a discovery-validation mass-spectrometry based pipeline to identify a set of proteins that are regulated in serum of patients with cervical intraepithelial neoplasia (CIN) and squamous cell cervical cancer using isobaric Tags for Relative and Absolute Quantitation (iTRAQ®), label-free s...
ORGANISM(S): Homo sapiens (Human) 
2014-10-06 | PXD001055 | Pride
Alpha-1-acid-glycoprotein Protein panel Alpha-1-antitrypsin Vitamin d-binding protein Intraepithelial neoplasia Haptoglobin Serotransferrin Alpha-2-hs-glycoprotein Cervical cancer Lc-ms/ms 
UnknownRobust, reproducible and quantitative analysis of thousands of proteomes by micro-flow LC-MS/MS.
Not available
S-EPMC6952431 | biostudies-literature
UnknownAn integrated encyclopedia of DNA elements in the human genome.
Not available
S-EPMC3439153 | biostudies-literature
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