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ProteomicsFarnesylated Heat Shock Protein 40 is a component of membrane-bound RISC in Arabidopsis
ARGONAUTE1 (AGO1) binds directly to small regulatory RNA and is a key effector protein of post-transcriptional gene silencing mediated by microRNA (miRNA) and small interfering RNA (siRNA). The formation of an RNA induced silencing complex (RISC) of AGO1 and small RNA requires the function of the He...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2018-09-10 | PXD010197 | Pride
Chaperones Small rna Farnesylation Polysomes. Argonaute Rough er 
TranscriptomicsMembrane association and activity of rough endoplasmic reticulum associated RISC requires farnesylated chaperones
Aim and experimental set-up: Sequencing of small RNA from total RNA fractions. The aim of this experiment was to compare profiles of miRNA expression between the following genetic backgrounds: Col-0 (wild type), era1-2 (farnesyl transferase mutant), j2-2/j3-2 + pJ3:J3 (j2/j3 double knockout expres...
ORGANISM(S): Arabidopsis thaliana 
2016-07-31 | E-MTAB-3736 | biostudies-arrayexpress
ProteomicsA YTHDF-PABP axis is required for m6A-mediated organogenesis in plants
N6-methyladenosine (m6A) in mRNA is key to eukaryotic gene regulation. Many m6A functions involve RNA-binding proteins that recognize m6A via a YT521-B Homology (YTH) domain. YTH domain proteins contain long intrinsically disordered regions (IDRs) that may mediate phase separation and interaction wi...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2023-11-28 | PXD045801 | Pride
Arabidopsis Plants M6a Ect2 
ProteomicsALBA proteins facilitate cytoplasmic YTHDF-mediated reading of m6A in plants
N6-methyladenosine (m6A) exerts many of its regulatory effects on eukaryotic mRNAs by recruiting cytoplasmic YT521-B homology domain family (YTHDF) proteins. Here, we show that in Arabidopsis, the interaction between m6A and the major YTHDF protein ECT2 also involves the mRNA-binding ALBA protein fa...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2024-11-18 | PXD052232 | Pride
Arabidopsis Ythdf M6a Alba 
Transcriptomicscomparison of transcriptomes in mad mutants-Transcriptome analysis of miRNA action deficient mutants
rs10-04_mad - comparison of transcriptomes in mad mutants - What gene sets are differentially expressed in mad mutants? - Seeds of GFP171.1 (parental line), mad1, mad2, mad3, mad6 and dcl1-12 were sterilized and germinated on Murashige/Skoog medium with 0.9% agar. Plates were stratified at 4C in the...
ORGANISM(S): Arabidopsis thaliana 
2011-09-14 | E-GEOD-30477 | biostudies-arrayexpress
TranscriptomicsComparison of IND induced vs. mock treated seedlings: Transcriptome profiling upon induction of the transcription factor IND
rs10-03_ind - comparison of ind induced vs mock treated seedlings - Identification of miRNAs regulated by IND - Transgenic seeds containing an IND transgene under the control of a dexamethasone inducible promoter were germinated and grown in liquid Murashige/Skoog medium under a 16h light/8h darknes...
ORGANISM(S): Arabidopsis thaliana 
2011-05-12 | E-GEOD-28898 | biostudies-arrayexpress
ProteomicsEvidence for an RNAi-independent role of DICER-LIKE2 in conferring growth inhibition and basal antiviral resistance
Higher plants encode four or five DICER-LIKE (DCL) enzymes responsible for the production of small non-coding RNAs which function in RNA interference (RNAi). Different RNAi pathways in plants effect transposon silencing, antiviral defense and endogenous gene regulation. DCL2 acts genetically redunda...
ORGANISM(S): Arabidopsis Thaliana 
2024-07-26 | PXD046163 | panorama
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