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GenomicsGenome-wide DNA methylation profiles of non-small cell lung carcinomas and control lung tissues
Purpose: Generate genome-wide methylation profiles of non-small cell lung carcinomas (NSCLC) and their matching lung tissues for detection of hypermethylated and hypomethylated regions present in the tumors. Methods: MethylCapture followed by next-generation sequencing (Illumina GAIIx) of 7 nsclc tu...
ORGANISM(S): Homo sapiens 
2012-10-05 | E-GEOD-41343 | biostudies-arrayexpress
TranscriptomicsDeciphering the RNA landscape by RNAome sequencing [Seq]
Current RNA expression profiling methods rely on enrichment steps for specific RNA classes, thereby not detecting all RNA species in an unperturbed manner. We report strand-specific RNAome sequencing that determines expression of small and large RNAs from ribosomal RNA-depleted total RNA in a single...
ORGANISM(S): Mus musculus 
2015-04-01 | E-GEOD-48083 | biostudies-arrayexpress
TranscriptomicsDeciphering the RNA landscape by RNAome sequencing [Affymetrix]
Current RNA expression profiling methods rely on enrichment steps for specific RNA classes, thereby not detecting all RNA species in an unperturbed manner. We report strand-specific RNAome sequencing that determines expression of small and large RNAs from ribosomal RNA-depleted total RNA in a single...
ORGANISM(S): Mus musculus 
2015-04-01 | E-GEOD-47888 | biostudies-arrayexpress
ProteomicsPU1 interactome in human monocyte derived dendritic cells
Human monocyte-derived DC were cultured at a density of 0.5x10E6 cells/ml in RPMI-1640 supplemented with 10% FCS, 1% Pen/Strep, 10 ng/ml GM-CSF and 10 ng/ml IL4. At day 7, they were stimulated or not with 500 ng/ml LPS. Cells were collected at day 10. Whole cell lystaes from 2x10E8 cells were used f...
ORGANISM(S): Homo Sapiens 
2018-11-30 | MSV000083175 | MassIVE
ProteomicsPU.1 interactome mouse bone marrow derived dendritic cells
Mouse bone marrow derived dendritic cells were generated by culturing bone marrow cells at a density of 0.5x10E6 cells/ml in RPMI-1640 supplemented with 5% FCS, 1% Pen/Strep, 5microM 2-mercaptoethanol, 20ng/ml GM-CSF. At day 7 dendritic cells were stimulated or not with 500 ng/ml LPS, and collected ...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2018-11-30 | MSV000083176 | MassIVE
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