Sort   by:  
 Page size 
ProteomicsQuantitative Analysis of Samples Labeled with Multiplex iqPIR Cross-Linkers
The isobaric quantitative protein interaction reporter (iqPIR) has now been extended to six channels, enabling quantitation of cross-links from six different samples simultaneously. To analyze data for such cross-linkers, the two-channel mode of quantitative analysis was extended to accommodate any...
ORGANISM(S): Homo sapiens (Human) 
2023-03-11 | PXD025796 | Pride
Quantitative analysis Mass spectrometry Crosslinking Multiplex iqpir 
ProteomicsMultiplexed isobaric quantitative cross-linking reveals drug-induced interactome changes in breast cancer cells
In vivo application of an extended set of six iqPIR reagents (6-plex iqPIR) allowing multiplexed quantitative interactome analysis of up to six biological samples in a single LC-MS acquisition. Multiplexed iqPIR is demonstrated on MCF-7 breast cancer cells treated with five different Hsp90 inhibito...
ORGANISM(S): Homo sapiens (Human) 
2023-03-11 | PXD029350 | Pride
Cross-linking Mass spectrometry Breast cancer 
ProteomicsChemical cross-linking mass spectrometry for systems structural biology in mouse heart tissue.
While modern structural biology technologies have greatly expanded the size and type of protein complexes that can now be studied, the ability to derive large-scale structural information on proteins and complexes as they exist within tissues is practically non-existent. Many protein properties and...
ORGANISM(S): Mus musculus (Mouse) 
2017-11-30 | PXD007673 | Pride
Mass spectrometry Structural biology Mouse Heart Protein-protein interactions Cross-linking 
ProteomicsAnalysis of bilirubin-induced bovine serum albumin stability using protein denaturation and quantitative cross-linking mass spectrometry
We report the combination of protein-denaturation stability principles with quantitative cross-linking mass spectrometry using isobaric quantitative protein interaction reporter technologies. This method enables the evaluation of ligand-induced protein engagement through analysis of cross-link rela...
ORGANISM(S): Bos taurus (Bovine) 
2024-07-03 | PXD036649 | Pride
Quantitative cross-linking Protein stability Mass spectrometry 
ProteomicsCellular interactome dynamics during paclitaxel treatment
Cell cycle inhibitors, including paclitaxel, are among the most widely used and effective cancer therapies. However, several challenges limit the success of paclitaxel including drug resistance and toxic side effects. Paclitaxel is thought to act primarily by stabilizing microtubules and locking cel...
ORGANISM(S): Homo sapiens (Human) 
2019-11-21 | PXD015751 | Pride
Mitotic inhibitor Chemical cross-linking 
ProteomicsMultiplexed isobaric quantitative Protein Interaction Reporter (iqPIR) cross-linking on standard proteins
Initial demonstration of multiplexed isobaric quantitative Protein Interaction Reporter technology using two multiplexed samples consisting of 5 cross-linked standard proteins including alcohol dehydrogenase 1 (ADH1_YEAST), albumin (ALBU_BOVIN), cytochrome c (CYC_BOVIN), hemoglobin (HBA_HUMAN), and ...
ORGANISM(S): Bos taurus (Bovine) Homo sapiens (Human) Saccharomyces cerevisiae (Baker's yeast) Equus caballus (Horse) 
2023-03-11 | PXD026037 | Pride
Multiplexed quantitation Cross-linking mass spectrometry 
ProteomicsApplication of harmonic FT-ICR-MS signal acquisition for improved proteome research
Proteomics experiments benefit from having both high resolving power and high scan speed. Harmonic detection provides a method to obtain twice the resolution for the same data acquisition period, or the same resolution in half the data acquisition period. We show that in a complex hela cell lysate s...
ORGANISM(S): Homo sapiens (Human) 
2022-08-12 | PXD022838 | Pride
Lc-ms/ms Fticr 
ProteomicsQuantitative cross-linking analysis of chemoresistant HeLa cells
Chemoresistance is a common mode of therapy failure for many cancers. Tumors develop resistance to chemotherapeutics through a variety of mechanisms, with proteins serving pivotal roles. Changes in protein conformations and interactions affect the cellular response to environmental conditions cont...
ORGANISM(S): Homo sapiens (Human) 
2015-09-15 | PXD002378 | Pride
Multidrug resistant cancer Chemical cross-linking Mass spectrometry Protein interactions 
ProteomicsHeLa Cells Cross-Linked In Vivo versus as a Lysate
HeLa Cells were cross-linked with PIR either in vivo or as a lysate.
ORGANISM(S): Homo sapiens (Human) 
2019-07-12 | PXD013063 | Pride
Pir cross-linker xl-ms 
ProteomicsLarge-Scale Quantitative Cross-Linking and Mass Spectrometry Provides New Insight on Protein Conformational Plasticity within Organelles, Cells, and Tissues
Many proteins can exist in multiple conformational states in vivo to achieve distinct functional roles. These states include alternative conformations, variable PTMs, and association with interacting protein, nucleotide, and ligand partners. Quantitative chemical cross-linking of live cells, organel...
ORGANISM(S): Mus musculus (Mouse) 
2026-01-12 | PXD058490 | Pride
M.musculus qxl-ms 
Sort   by:  
 Page size