Recombinant proteins were mixed and then crosslinked either with DMTMM or by SDA and UV exposure. Complexes were resolved by SDS-PAGE, excised and tryptic peptides identified by MS.
Metabolic networks are interconnected and influence diverse cellular processes. The protein-metabolite interactions that mediate these networks are frequently low affinity and challenging to systematically discover. We developed mass spectrometry integrated with equilibrium dialysis for the disco...
