Sort   by:  
 Page size 
TranscriptomicsUpstream Anti-sense Promoters Act as Local Enhancers of Mammalian Protein-coding Genes
Anti-sense transcription originating upstream of mammalian protein-coding genes is a well-documented phenomenon, but remarkably little is known about the function or regulation of these anti-sense promoters or the non-coding RNAs they generate. Here we define at nucleotide resolution the divergent t...
ORGANISM(S): Mus musculus 
2015-06-23 | E-GEOD-62151 | biostudies-arrayexpress
GenomicsInterrelationships between replication infidelity and genome composition
An enduring issue in evolutionary and cancer biology is how replication infidelity influences genome composition and vice versa. Here we examine this issue by sequencing the genomes of diploid budding yeast strains that are either mismatch repair (MMR) proficient or deficient and encode wild type or...
ORGANISM(S): Saccharomyces cerevisiae 
2014-08-25 | E-GEOD-56939 | biostudies-arrayexpress
TranscriptomicsStable pausing by RNA polymerase II provides an opportunity to target and integrate regulatory signals
Metazoan gene expression is often regulated after the recruitment of RNA polymerase II (Pol II) to promoters, through the controlled release of promoter-proximally paused Pol II into productive RNA synthesis. Despite the prevalence of paused Pol II, very little is known about the dynamics of these e...
ORGANISM(S): Drosophila melanogaster 
2013-10-31 | E-GEOD-49078 | biostudies-arrayexpress
TranscriptomicsTracking replication enzymology in vivo by genome-wide mapping of ribonucleotide incorporation
Multiple DNA polymerases are needed to replicate genetic information. Here we describe the use of ribonucleotide incorporation as a biomarker of replication enzymology in vivo. We find that ribonucleotides are incorporated into the yeast nuclear genome in replicase specific and strand-specific patte...
ORGANISM(S): Saccharomyces cerevisiae 
2015-01-26 | E-GEOD-62181 | biostudies-arrayexpress
GenomicsTRIM28 regulates RNA polymerase II promoter proximal pausing and entry into processive elongation
Analysis of total PolII and Ser2-phosphorylated PolII genomic occupancy in control-transfected (WT) or Trim28-knockdown (Trim28-KD) embryonic stem cells. Embryonic stem cells were transfected with luciferase siRNA (WT) or Trim28 siRNA (Trim28 KD) in duplicate experiments. 48 hrs after transfection, ...
ORGANISM(S): Mus musculus 
2014-07-28 | E-GEOD-48253 | biostudies-arrayexpress
Sort   by:  
 Page size