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TranscriptomicsDetermine whether Streptomyces venezuelae protein SepH (vnz_27360) binds DNA and where
SepH was identified having a potential role in sporulation following ChIP-seq analysis that showed the sepH gene is under the control of the WhiA and WhiB transcription factors (Bush et al., 2013; Bush et al., 2016). Since SepH has a helix-turn-helix motif, this ChIP-Seq experiment was carried out t...
ORGANISM(S): Streptomyces venezuelae 
2020-09-30 | E-MTAB-9064 | biostudies-arrayexpress
TranscriptomicsWhiA and WhiB of Streptomyces venezuelae depend on each other for binding DNA
WhiB regulon, as determined by ChIP-Seq turned out to be identical to the WhiA regulon leading to the speculation that the two proteins might be required to work together to bind DNA. In this experiment WhiA ChIP-Seq was done in a whiB deletion background and, WhiB ChIP-Seq was done in a whiA deleti...
ORGANISM(S): Streptomyces venezuelae 
2021-07-01 | E-MTAB-10673 | biostudies-arrayexpress
TranscriptomicsChIP-Seq to determine binding sites of transcription factor BldO in the genome of Streptomyces venezuelae
The transcription factor BldO was identified as a target of WhiAB in an earlier ChIP-Seq experiment. This experiment was conducted to determine the targets of BldO in the Streptomyces venezuelae genome.
ORGANISM(S): Streptomyces venezuelae 
2021-03-31 | E-MTAB-10250 | biostudies-arrayexpress
TranscriptomicsChIP-Seq to determine the binding sites of the transcription factor WhiB in the genome of Streptomyces venezuelae
WhiB is the founding member of a family of proteins (the WhiB-like [Wbl] family) that carry a [4Fe-4S] iron-sulfur cluster. It is essential for the process of developmentally controlled cell division leading to sporulation in Streptomyces species. This ChIP-Sea was carried out to determine unambiguo...
ORGANISM(S): Streptomyces venezuelae 
2021-06-25 | E-MTAB-10648 | biostudies-arrayexpress
TranscriptomicsMutation of the cysteine residues that coordinate the WhiB [4Fe-4S] cluster prevents in vivo DNA binding by both WhiB and WhiA
The purpose of this experiment was to show that the iron-sulphur cluster ([4Fe-4S]) of whiB is essential for the DNA binding by WhiB and WhiA. The \\"serine version of whiB\\" mentioned below is a whiB in which the 4 cysteine residues which coordinate the [4Fe-4S] cluster were mutated to serines. Ch...
ORGANISM(S): Streptomyces venezuelae 
2021-08-16 | E-MTAB-10818 | biostudies-arrayexpress
TranscriptomicsTranscriptional profiling of the developmental gene whiB deletion mutant of Streptomyces venezuelae ATCC 10712 at 7 time points from 8 to 20 hours of growth
WhiB is the founding member of a family of proteins (the WhiB-like [Wbl] family) that carry a [4Fe-4S] iron-sulfur cluster and play key roles in diverse aspects of the biology of actinomycetes, including pathogenesis, antibiotic resistance, and the control of development. In Streptomyces, WhiB is es...
ORGANISM(S): Streptomyces venezuelae 
2021-06-30 | E-MTAB-10631 | biostudies-arrayexpress
TranscriptomicsTranscriptional profiling of the developmental gene whiA deletion mutant of Streptomyces venezuelae ATCC 10712 at 7 time points from 8 to 20 hours of growth.
WhiA is an unusual transcriptional regulator found in Streptomyces species related to eukaryotic homing endonucleases. It plays a central role in sporulation in these bacteria. The aim of this transcription profiling experiment was to measure genome wide transcript levels in the wild type and the wh...
ORGANISM(S): Streptomyces venezuelae 
2020-12-11 | E-MTAB-9728 | biostudies-arrayexpress
ProteomicsDisordered region encodes α-crystallin chaperone activity toward lens client γD-crystallin
Small heat-shock proteins (sHSPs) are a widely expressed family of ATP-independent molecular chaperones that are among the first responders to cellular stress. Mechanisms by which sHSPs delay aggregation of client proteins remain undefined. sHSPs have high intrinsic disorder content of up to ~60% an...
ORGANISM(S): Escherichia coli 
2023-01-31 | PXD035668 | Pride
Hspb5 Small heat shock proteins Crosslinking Bpa 
TranscriptomicsIdentification of CPK5ac and CPK11ac early target genes in Arabidopsis mesophyll cells
The goal of this experiment was to identify the early target genes of constitutively activated CPK5ac and CPK11ac in Arabidopsis mesophyll cells that are involved in early flagellin responses important for plant innate immunity. We used the microarrays to detail the global program of gene expression...
ORGANISM(S): Arabidopsis thaliana 
2010-03-18 | E-GEOD-16471 | biostudies-arrayexpress
TranscriptomicsIdentification of early flg22 responsive genes in Arabidopsis mesophyll cells
The goal of this experiment was to identify the early responsive genes activated by the 22 amino acid peptide of bacterial flagellin (flg22) in Arabidopsis mesophyll cells that are involved in the initial responses important for plant innate immunity. We used the microarrays to detail the global pro...
ORGANISM(S): Arabidopsis thaliana 
2010-03-18 | E-GEOD-16472 | biostudies-arrayexpress
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