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Reference design (using Cy5 labelled genomic DNA as the reference) to compare two genotypes: wild-type W50 vs an isogenic FeoB1 mutant W50FB1. Six biological replicates of both W50 and W50FB1, grown in continuous culture.
ORGANISM(S): Porphyromonas gingivalis 
Porphyromonas gingivalis is a major pathogen associated with the microbial biofilm-mediated disease chronic periodontitis. P. gingivalis has an obligate requirement for iron and protoporphyrin IX which it satisfies by transporting heme and iron liberated from the human host. The level of cellular ir...
ORGANISM(S): Porphyromonas gingivalis ATCC 33277 
Human satellite cells were isolated from muscle biopsies and expanded in vitro. Cells were induced to differentiate into branched multinucleated myotubes by serum depletion and were harvested from three independent cultures after 72 hours. Total RNA was isolated, amplified, labelled and hybridised...
ORGANISM(S): Homo sapiens 
The aim of this experiment is to ascertain which genes have their expression influenced by PG1432. PG1432 encodes a putative sensor histidine kinase which is part of two component signal transduction that was highly up-regulated in P. gingivalis mature biofilm cells. PG1432 was disrupted in P. gingi...
ORGANISM(S): Porphyromonas gingivalis 
Genomic imprinting results in the preferential expression of the paternal, or maternal allele of certain genes. We have performed a genome-wide characterization of imprinting in the mouse embryonic and adult brain using F1 hybrid mice generated from reciprocal crosses of CASTEiJ and C57BL/6J mice. ...
ORGANISM(S): Mus musculus 
Cancer is driven by mutation. Using Agilent exome hybridisation capture and Illumina GA massively parallel sequencing technology, we aim to sequence ~1600 microRNAs plus the protein coding genome of 25 matched human renal cancer samples. Bespoke algorithms are being developed to identify the somatic...
Sequencing data from natching Pancreatic Carcinoma samples
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