Transcription profiling of Porphyromonas gingivalis wild type and mutant PG1432::ermF to identify genes which have their expression influenced by PG1432

ABSTRACT: The aim of this experiment is to ascertain which genes have their expression influenced by PG1432. PG1432 encodes a putative sensor histidine kinase which is part of two component signal transduction that was highly up-regulated in P. gingivalis mature biofilm cells. PG1432 was disrupted in P. gingivalis W50 by insertion of ermF cassette. The strain W50 PG1432 mutant was designated ECR222. Two independent replicates of each strain were grown for 30 days in continuous culture in chemostats using brain heart infusion media supplemented with 0.5 mg mL-1 cysteine and 5 �~A�g mL-1 hemin. Although strain W50 formed sufficient biofilm to use for total RNA harvest and microarray analysis, strain ERC222 formed only sparse biofilm showing that disruption of PG1432 impaired biofilm formation by t

ORGANISM(S): Porphyromonas gingivalis

SUBMITTER: Alvin Lo 

PROVIDER: E-TABM-546 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress