Sort   by:  
 Page size 
ProteomicsMining novel gene targets for improving tolerance to furfural and acetic acid in Yarrowia lipolytica using whole-genome clustered regularly interspaced short palindromic repeats interference library
In this study, a whole-genome CRISPRi library was developed in Y. lipolytica and applied for enforcing the tolerance to furfural and acetic acid. Several novel gene targets were discovered through NGS before and after screening. And to analyze the tolerance mechanism caused by transcriptional repres...
ORGANISM(S): Yarrowia Lipolytica 
2024-03-31 | PXD051116 |
ProteomicsGenome-scale CRISPRi screen identifies pcnB repression conferring overproduction of free fatty acids in Escherichia coli
In this study, we carried out genome-scale CRISPRi modulation combining fluorescence-activated cell sorting (FACS) and next-generation sequencing (NGS) to identify genetic determinants for FFAs overproduction in Escherichia coli. The pcnBi (AP) strain that repressed the expression of pcnB produced 2...
ORGANISM(S): Escherichia Coli 
PXD052390 |
ProteomicsGenome-wide targets identification for high-titer production of free fatty acids in Escherichia coli
We employed omics analyses to identify potential targets in multiple cellular processes, enabling systematical discovery of beneficial chromosomal gene targets that can be engineered to optimize free fatty acids (FFAs) production in Escherichia coli.
ORGANISM(S): Escherichia Coli 
PXD017890 |
Sort   by:  
 Page size