Flag antibody was used for immunoprecipitation, and cd229-OE partial binding protein was searched by protein spectrum in CD229-OE multiple myeloma cell line, and its interaction was analyzed.
We identify the Kcr sites on histones that are targeted by CDYL in ADPKD cells (WT9-12 cells). We chose to knock out CDYL in WT 9-12 cells (CDYL KO) through CRISPR/Cas9-mediated gene editing to increase the overall levels of histone Kcr. We quantified Kcr modifications on different histone residues ...
